Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food

We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using...

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Autores principales: Peng, Juan, Song, Shanshan, Xu, Liguang, Ma, Wei, Liu, Liqiang, Kuang, Hua, Xu, Chuanlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734466/
https://www.ncbi.nlm.nih.gov/pubmed/23880725
http://dx.doi.org/10.3390/ijerph10072897
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author Peng, Juan
Song, Shanshan
Xu, Liguang
Ma, Wei
Liu, Liqiang
Kuang, Hua
Xu, Chuanlai
author_facet Peng, Juan
Song, Shanshan
Xu, Liguang
Ma, Wei
Liu, Liqiang
Kuang, Hua
Xu, Chuanlai
author_sort Peng, Juan
collection PubMed
description We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.
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spelling pubmed-37344662013-08-06 Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food Peng, Juan Song, Shanshan Xu, Liguang Ma, Wei Liu, Liqiang Kuang, Hua Xu, Chuanlai Int J Environ Res Public Health Article We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content. MDPI 2013-07-12 2013-07 /pmc/articles/PMC3734466/ /pubmed/23880725 http://dx.doi.org/10.3390/ijerph10072897 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Peng, Juan
Song, Shanshan
Xu, Liguang
Ma, Wei
Liu, Liqiang
Kuang, Hua
Xu, Chuanlai
Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
title Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
title_full Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
title_fullStr Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
title_full_unstemmed Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
title_short Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
title_sort development of a monoclonal antibody-based sandwich elisa for peanut allergen ara h 1 in food
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734466/
https://www.ncbi.nlm.nih.gov/pubmed/23880725
http://dx.doi.org/10.3390/ijerph10072897
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