Cargando…
Targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet
The metabolic syndrome is a collection of risk factors including obesity, insulin resistance and hepatic steatosis, which occur together and increase the risk of diseases such as diabetes, cardiovascular disease and cancer. In spite of intense research, the complex etiology of insulin resistance and...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Molecular Biology Organization
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734509/ https://www.ncbi.nlm.nih.gov/pubmed/23860498 http://dx.doi.org/10.1038/msb.2013.36 |
_version_ | 1782279550438211584 |
---|---|
author | Sabidó, Eduard Wu, Yibo Bautista, Lucia Porstmann, Thomas Chang, Ching-Yun Vitek, Olga Stoffel, Markus Aebersold, Ruedi |
author_facet | Sabidó, Eduard Wu, Yibo Bautista, Lucia Porstmann, Thomas Chang, Ching-Yun Vitek, Olga Stoffel, Markus Aebersold, Ruedi |
author_sort | Sabidó, Eduard |
collection | PubMed |
description | The metabolic syndrome is a collection of risk factors including obesity, insulin resistance and hepatic steatosis, which occur together and increase the risk of diseases such as diabetes, cardiovascular disease and cancer. In spite of intense research, the complex etiology of insulin resistance and its association with the accumulation of triacylglycerides in the liver and with hepatic steatosis remains not completely understood. Here, we performed quantitative measurements of 144 proteins involved in the insulin-signaling pathway and central metabolism in liver homogenates of two genetically well-defined mouse strains C57BL/6J and 129Sv that were subjected to a sustained high-fat diet. We used targeted mass spectrometry by selected reaction monitoring (SRM) to generate accurate and reproducible quantitation of the targeted proteins across 36 different samples (12 conditions and 3 biological replicates), generating one of the largest quantitative targeted proteomics data sets in mammalian tissues. Our results revealed rapid response to high-fat diet that diverged early in the feeding regimen, and evidenced a response to high-fat diet dominated by the activation of peroxisomal β-oxidation in C57BL/6J and by lipogenesis in 129Sv mice. |
format | Online Article Text |
id | pubmed-3734509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | European Molecular Biology Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-37345092013-08-06 Targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet Sabidó, Eduard Wu, Yibo Bautista, Lucia Porstmann, Thomas Chang, Ching-Yun Vitek, Olga Stoffel, Markus Aebersold, Ruedi Mol Syst Biol Article The metabolic syndrome is a collection of risk factors including obesity, insulin resistance and hepatic steatosis, which occur together and increase the risk of diseases such as diabetes, cardiovascular disease and cancer. In spite of intense research, the complex etiology of insulin resistance and its association with the accumulation of triacylglycerides in the liver and with hepatic steatosis remains not completely understood. Here, we performed quantitative measurements of 144 proteins involved in the insulin-signaling pathway and central metabolism in liver homogenates of two genetically well-defined mouse strains C57BL/6J and 129Sv that were subjected to a sustained high-fat diet. We used targeted mass spectrometry by selected reaction monitoring (SRM) to generate accurate and reproducible quantitation of the targeted proteins across 36 different samples (12 conditions and 3 biological replicates), generating one of the largest quantitative targeted proteomics data sets in mammalian tissues. Our results revealed rapid response to high-fat diet that diverged early in the feeding regimen, and evidenced a response to high-fat diet dominated by the activation of peroxisomal β-oxidation in C57BL/6J and by lipogenesis in 129Sv mice. European Molecular Biology Organization 2013-07-16 /pmc/articles/PMC3734509/ /pubmed/23860498 http://dx.doi.org/10.1038/msb.2013.36 Text en Copyright © 2013, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission. |
spellingShingle | Article Sabidó, Eduard Wu, Yibo Bautista, Lucia Porstmann, Thomas Chang, Ching-Yun Vitek, Olga Stoffel, Markus Aebersold, Ruedi Targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet |
title | Targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet |
title_full | Targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet |
title_fullStr | Targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet |
title_full_unstemmed | Targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet |
title_short | Targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet |
title_sort | targeted proteomics reveals strain-specific changes in the mouse insulin and central metabolic pathways after a sustained high-fat diet |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734509/ https://www.ncbi.nlm.nih.gov/pubmed/23860498 http://dx.doi.org/10.1038/msb.2013.36 |
work_keys_str_mv | AT sabidoeduard targetedproteomicsrevealsstrainspecificchangesinthemouseinsulinandcentralmetabolicpathwaysafterasustainedhighfatdiet AT wuyibo targetedproteomicsrevealsstrainspecificchangesinthemouseinsulinandcentralmetabolicpathwaysafterasustainedhighfatdiet AT bautistalucia targetedproteomicsrevealsstrainspecificchangesinthemouseinsulinandcentralmetabolicpathwaysafterasustainedhighfatdiet AT porstmannthomas targetedproteomicsrevealsstrainspecificchangesinthemouseinsulinandcentralmetabolicpathwaysafterasustainedhighfatdiet AT changchingyun targetedproteomicsrevealsstrainspecificchangesinthemouseinsulinandcentralmetabolicpathwaysafterasustainedhighfatdiet AT vitekolga targetedproteomicsrevealsstrainspecificchangesinthemouseinsulinandcentralmetabolicpathwaysafterasustainedhighfatdiet AT stoffelmarkus targetedproteomicsrevealsstrainspecificchangesinthemouseinsulinandcentralmetabolicpathwaysafterasustainedhighfatdiet AT aebersoldruedi targetedproteomicsrevealsstrainspecificchangesinthemouseinsulinandcentralmetabolicpathwaysafterasustainedhighfatdiet |