Cargando…
Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase
The ultimate goal of this research is to construct a new direct CO(2) fixation system using photosystems in living algae. Here, we report light-driven formate production from CO(2) by using cyanobacterial photosystem I (PS I). Formate, a chemical hydrogen carrier and important industrial material, c...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3735542/ https://www.ncbi.nlm.nih.gov/pubmed/23936519 http://dx.doi.org/10.1371/journal.pone.0071581 |
Sumario: | The ultimate goal of this research is to construct a new direct CO(2) fixation system using photosystems in living algae. Here, we report light-driven formate production from CO(2) by using cyanobacterial photosystem I (PS I). Formate, a chemical hydrogen carrier and important industrial material, can be produced from CO(2) by using the reducing power and the catalytic function of formate dehydrogenase (FDH). We created a bacterial FDH mutant that experimentally switched the cofactor specificity from NADH to NADPH, and combined it with an in vitro-reconstituted cyanobacterial light-driven NADPH production system consisting of PS I, ferredoxin (Fd), and ferredoxin-NADP(+)-reductase (FNR). Consequently, light-dependent formate production under a CO(2) atmosphere was successfully achieved. In addition, we introduced the NADPH-dependent FDH mutant into heterocysts of the cyanobacterium Anabaena sp. PCC 7120 and demonstrated an increased formate concentration in the cells. These results provide a new possibility for photo-biological CO(2) fixation. |
---|