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Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase
The ultimate goal of this research is to construct a new direct CO(2) fixation system using photosystems in living algae. Here, we report light-driven formate production from CO(2) by using cyanobacterial photosystem I (PS I). Formate, a chemical hydrogen carrier and important industrial material, c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3735542/ https://www.ncbi.nlm.nih.gov/pubmed/23936519 http://dx.doi.org/10.1371/journal.pone.0071581 |
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author | Ihara, Masaki Kawano, Yusuke Urano, Miho Okabe, Ayako |
author_facet | Ihara, Masaki Kawano, Yusuke Urano, Miho Okabe, Ayako |
author_sort | Ihara, Masaki |
collection | PubMed |
description | The ultimate goal of this research is to construct a new direct CO(2) fixation system using photosystems in living algae. Here, we report light-driven formate production from CO(2) by using cyanobacterial photosystem I (PS I). Formate, a chemical hydrogen carrier and important industrial material, can be produced from CO(2) by using the reducing power and the catalytic function of formate dehydrogenase (FDH). We created a bacterial FDH mutant that experimentally switched the cofactor specificity from NADH to NADPH, and combined it with an in vitro-reconstituted cyanobacterial light-driven NADPH production system consisting of PS I, ferredoxin (Fd), and ferredoxin-NADP(+)-reductase (FNR). Consequently, light-dependent formate production under a CO(2) atmosphere was successfully achieved. In addition, we introduced the NADPH-dependent FDH mutant into heterocysts of the cyanobacterium Anabaena sp. PCC 7120 and demonstrated an increased formate concentration in the cells. These results provide a new possibility for photo-biological CO(2) fixation. |
format | Online Article Text |
id | pubmed-3735542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37355422013-08-09 Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase Ihara, Masaki Kawano, Yusuke Urano, Miho Okabe, Ayako PLoS One Research Article The ultimate goal of this research is to construct a new direct CO(2) fixation system using photosystems in living algae. Here, we report light-driven formate production from CO(2) by using cyanobacterial photosystem I (PS I). Formate, a chemical hydrogen carrier and important industrial material, can be produced from CO(2) by using the reducing power and the catalytic function of formate dehydrogenase (FDH). We created a bacterial FDH mutant that experimentally switched the cofactor specificity from NADH to NADPH, and combined it with an in vitro-reconstituted cyanobacterial light-driven NADPH production system consisting of PS I, ferredoxin (Fd), and ferredoxin-NADP(+)-reductase (FNR). Consequently, light-dependent formate production under a CO(2) atmosphere was successfully achieved. In addition, we introduced the NADPH-dependent FDH mutant into heterocysts of the cyanobacterium Anabaena sp. PCC 7120 and demonstrated an increased formate concentration in the cells. These results provide a new possibility for photo-biological CO(2) fixation. Public Library of Science 2013-08-06 /pmc/articles/PMC3735542/ /pubmed/23936519 http://dx.doi.org/10.1371/journal.pone.0071581 Text en © 2013 Ihara et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ihara, Masaki Kawano, Yusuke Urano, Miho Okabe, Ayako Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase |
title | Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase |
title_full | Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase |
title_fullStr | Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase |
title_full_unstemmed | Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase |
title_short | Light Driven CO(2) Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase |
title_sort | light driven co(2) fixation by using cyanobacterial photosystem i and nadph-dependent formate dehydrogenase |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3735542/ https://www.ncbi.nlm.nih.gov/pubmed/23936519 http://dx.doi.org/10.1371/journal.pone.0071581 |
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