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Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA
In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a pre-Replicative Complex (pre-RC) with a MCM2-7 double-hexamer encircling DNA. Using purified components in the presence of ATPγS, we have captured in vitro an intermediate in pre-RC ass...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3735830/ https://www.ncbi.nlm.nih.gov/pubmed/23851460 http://dx.doi.org/10.1038/nsmb.2629 |