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Gastrointestinal transit measurements in mice with (99m)Tc-DTPA-labeled activated charcoal using NanoSPECT-CT
BACKGROUND: Gastrointestinal (GI) disorders are commonly associated with chronic conditions such as diabetes, obesity, and hypertension. Direct consequences are obstipation or diarrhea as opposite aspects of the irritable bowel syndrome, and more indirectly, alteration of appetite, feeling of fullne...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3737085/ https://www.ncbi.nlm.nih.gov/pubmed/23915679 http://dx.doi.org/10.1186/2191-219X-3-60 |
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author | Padmanabhan, Parasuraman Grosse, Johannes Asad, Abu Bakar Md Ali Radda, George K Golay, Xavier |
author_facet | Padmanabhan, Parasuraman Grosse, Johannes Asad, Abu Bakar Md Ali Radda, George K Golay, Xavier |
author_sort | Padmanabhan, Parasuraman |
collection | PubMed |
description | BACKGROUND: Gastrointestinal (GI) disorders are commonly associated with chronic conditions such as diabetes, obesity, and hypertension. Direct consequences are obstipation or diarrhea as opposite aspects of the irritable bowel syndrome, and more indirectly, alteration of appetite, feeling of fullness, flatulence, bloatedness, and eventually leading to altered absorption of nutrients. Moreover, GI retention and passage times have been recognized as important factors in determining the release site and hence the bioavailability of orally administered drugs. To facilitate the understanding of physiological and pathological processes involved, it is necessary to monitor the gut motility in animal models. Here, we describe a method for studying the GI transit time using technetium-labeled activated charcoal diethylenetriaminepentaacetic acid ((99m)Tc-Ch-DTPA) detected by single-photon emission computed tomography (SPECT). METHODS: Tc-DTPA was adsorbed onto activated charcoal and administered orally to trypan blue-tainted (n = 4) 129SvEv mice (50 to 80 MBq/animal, n = 11). The exact distribution and movement of radioactivity in the gastrointestinal tract was measured at intervals of 1, 3, 6, 12, and 22 h by SPECT-CT. In addition, in order to validate the imaging of GI transient time, loperamide (0.25 mg/animal, n = 3) was used to delay the GI transit. RESULTS: The transit time measured as the peak radioactivity occurring in the rectum was 6 to 7 h after gavaging of (99m)Tc-Ch-DTPA. After 1 h, the bolus had passed into the small intestine and entered the cecum and the colon. At 6 and 8 h, the cecum, the ascending, transverse, and descending colon, and the rectum showed significant labeling. Several pellets were stored in the rectum for defecation. After 22 h, little activity remained in the stomach and none was detected in the transverse colon or other GI locations. In contrast, 6 h after administration of loperamide, only the cecum and part of the transverse colon were labeled. After 22 h, both structures retained significant amount of label. This delay has been verified by non-radiolabeled dye trypan blue GI measurements (n = 4). CONCLUSION: Here, we present the first non-invasive study of mouse GI transit time, allowing clear differentiation between vehicle- and loperamide-treated animals. This technique is useful for the investigation of GI motility in mice. |
format | Online Article Text |
id | pubmed-3737085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-37370852013-08-08 Gastrointestinal transit measurements in mice with (99m)Tc-DTPA-labeled activated charcoal using NanoSPECT-CT Padmanabhan, Parasuraman Grosse, Johannes Asad, Abu Bakar Md Ali Radda, George K Golay, Xavier EJNMMI Res Original Research BACKGROUND: Gastrointestinal (GI) disorders are commonly associated with chronic conditions such as diabetes, obesity, and hypertension. Direct consequences are obstipation or diarrhea as opposite aspects of the irritable bowel syndrome, and more indirectly, alteration of appetite, feeling of fullness, flatulence, bloatedness, and eventually leading to altered absorption of nutrients. Moreover, GI retention and passage times have been recognized as important factors in determining the release site and hence the bioavailability of orally administered drugs. To facilitate the understanding of physiological and pathological processes involved, it is necessary to monitor the gut motility in animal models. Here, we describe a method for studying the GI transit time using technetium-labeled activated charcoal diethylenetriaminepentaacetic acid ((99m)Tc-Ch-DTPA) detected by single-photon emission computed tomography (SPECT). METHODS: Tc-DTPA was adsorbed onto activated charcoal and administered orally to trypan blue-tainted (n = 4) 129SvEv mice (50 to 80 MBq/animal, n = 11). The exact distribution and movement of radioactivity in the gastrointestinal tract was measured at intervals of 1, 3, 6, 12, and 22 h by SPECT-CT. In addition, in order to validate the imaging of GI transient time, loperamide (0.25 mg/animal, n = 3) was used to delay the GI transit. RESULTS: The transit time measured as the peak radioactivity occurring in the rectum was 6 to 7 h after gavaging of (99m)Tc-Ch-DTPA. After 1 h, the bolus had passed into the small intestine and entered the cecum and the colon. At 6 and 8 h, the cecum, the ascending, transverse, and descending colon, and the rectum showed significant labeling. Several pellets were stored in the rectum for defecation. After 22 h, little activity remained in the stomach and none was detected in the transverse colon or other GI locations. In contrast, 6 h after administration of loperamide, only the cecum and part of the transverse colon were labeled. After 22 h, both structures retained significant amount of label. This delay has been verified by non-radiolabeled dye trypan blue GI measurements (n = 4). CONCLUSION: Here, we present the first non-invasive study of mouse GI transit time, allowing clear differentiation between vehicle- and loperamide-treated animals. This technique is useful for the investigation of GI motility in mice. Springer 2013-08-02 /pmc/articles/PMC3737085/ /pubmed/23915679 http://dx.doi.org/10.1186/2191-219X-3-60 Text en Copyright ©2013 Padmanabhan et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Padmanabhan, Parasuraman Grosse, Johannes Asad, Abu Bakar Md Ali Radda, George K Golay, Xavier Gastrointestinal transit measurements in mice with (99m)Tc-DTPA-labeled activated charcoal using NanoSPECT-CT |
title | Gastrointestinal transit measurements in mice with (99m)Tc-DTPA-labeled activated charcoal using NanoSPECT-CT |
title_full | Gastrointestinal transit measurements in mice with (99m)Tc-DTPA-labeled activated charcoal using NanoSPECT-CT |
title_fullStr | Gastrointestinal transit measurements in mice with (99m)Tc-DTPA-labeled activated charcoal using NanoSPECT-CT |
title_full_unstemmed | Gastrointestinal transit measurements in mice with (99m)Tc-DTPA-labeled activated charcoal using NanoSPECT-CT |
title_short | Gastrointestinal transit measurements in mice with (99m)Tc-DTPA-labeled activated charcoal using NanoSPECT-CT |
title_sort | gastrointestinal transit measurements in mice with (99m)tc-dtpa-labeled activated charcoal using nanospect-ct |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3737085/ https://www.ncbi.nlm.nih.gov/pubmed/23915679 http://dx.doi.org/10.1186/2191-219X-3-60 |
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