crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus

The Cas9-crRNA complex of the Streptococcus thermophilus DGCC7710 CRISPR3-Cas system functions as an RNA-guided endonuclease with crRNA-directed target sequence recognition and protein-mediated DNA cleavage. We show here that an additional RNA molecule, tracrRNA (trans-activating CRISPR RNA), co-pur...

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Detalles Bibliográficos
Autores principales: Karvelis, Tautvydas, Gasiunas, Giedrius, Miksys, Algirdas, Barrangou, Rodolphe, Horvath, Philippe, Siksnys, Virginijus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3737341/
https://www.ncbi.nlm.nih.gov/pubmed/23535272
http://dx.doi.org/10.4161/rna.24203

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3737341/
https://www.ncbi.nlm.nih.gov/pubmed/23535272
http://dx.doi.org/10.4161/rna.24203

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