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Density-dependent cooperative non-specific binding in solid-phase SELEX affinity selection
The non-specific binding of undesired ligands to a target is the primary factor limiting the enrichment of tight-binding ligands in affinity selection. Solution-phase non-specific affinity is determined by the free-energy of ligand binding to a single target. However, the solid-phase affinity might...
Autores principales: | Ozer, Abdullah, White, Brian S., Lis, John T., Shalloway, David |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3737557/ https://www.ncbi.nlm.nih.gov/pubmed/23737446 http://dx.doi.org/10.1093/nar/gkt477 |
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