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Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis
The West Nile virus (WNV) is an emerging infection of biodefense concern and there are no available treatments or vaccines. Here we used a high-throughput method based on a novel gene expression analysis, RNA-Seq, to give a global picture of differential gene expression by primary human macrophages...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738954/ https://www.ncbi.nlm.nih.gov/pubmed/23881275 http://dx.doi.org/10.3390/v5071664 |
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author | Qian, Feng Chung, Lisa Zheng, Wei Bruno, Vincent Alexander, Roger P. Wang, Zhong Wang, Xiaomei Kurscheid, Sebastian Zhao, Hongyu Fikrig, Erol Gerstein, Mark Snyder, Michael Montgomery, Ruth R. |
author_facet | Qian, Feng Chung, Lisa Zheng, Wei Bruno, Vincent Alexander, Roger P. Wang, Zhong Wang, Xiaomei Kurscheid, Sebastian Zhao, Hongyu Fikrig, Erol Gerstein, Mark Snyder, Michael Montgomery, Ruth R. |
author_sort | Qian, Feng |
collection | PubMed |
description | The West Nile virus (WNV) is an emerging infection of biodefense concern and there are no available treatments or vaccines. Here we used a high-throughput method based on a novel gene expression analysis, RNA-Seq, to give a global picture of differential gene expression by primary human macrophages of 10 healthy donors infected in vitro with WNV. From a total of 28 million reads per sample, we identified 1,514 transcripts that were differentially expressed after infection. Both predicted and novel gene changes were detected, as were gene isoforms, and while many of the genes were expressed by all donors, some were unique. Knock-down of genes not previously known to be associated with WNV resistance identified their critical role in control of viral infection. Our study distinguishes both common gene pathways as well as novel cellular responses. Such analyses will be valuable for translational studies of susceptible and resistant individuals—and for targeting therapeutics—in multiple biological settings. |
format | Online Article Text |
id | pubmed-3738954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-37389542013-08-09 Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis Qian, Feng Chung, Lisa Zheng, Wei Bruno, Vincent Alexander, Roger P. Wang, Zhong Wang, Xiaomei Kurscheid, Sebastian Zhao, Hongyu Fikrig, Erol Gerstein, Mark Snyder, Michael Montgomery, Ruth R. Viruses Article The West Nile virus (WNV) is an emerging infection of biodefense concern and there are no available treatments or vaccines. Here we used a high-throughput method based on a novel gene expression analysis, RNA-Seq, to give a global picture of differential gene expression by primary human macrophages of 10 healthy donors infected in vitro with WNV. From a total of 28 million reads per sample, we identified 1,514 transcripts that were differentially expressed after infection. Both predicted and novel gene changes were detected, as were gene isoforms, and while many of the genes were expressed by all donors, some were unique. Knock-down of genes not previously known to be associated with WNV resistance identified their critical role in control of viral infection. Our study distinguishes both common gene pathways as well as novel cellular responses. Such analyses will be valuable for translational studies of susceptible and resistant individuals—and for targeting therapeutics—in multiple biological settings. MDPI 2013-07-08 /pmc/articles/PMC3738954/ /pubmed/23881275 http://dx.doi.org/10.3390/v5071664 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Qian, Feng Chung, Lisa Zheng, Wei Bruno, Vincent Alexander, Roger P. Wang, Zhong Wang, Xiaomei Kurscheid, Sebastian Zhao, Hongyu Fikrig, Erol Gerstein, Mark Snyder, Michael Montgomery, Ruth R. Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis |
title | Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis |
title_full | Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis |
title_fullStr | Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis |
title_full_unstemmed | Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis |
title_short | Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis |
title_sort | identification of genes critical for resistance to infection by west nile virus using rna-seq analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738954/ https://www.ncbi.nlm.nih.gov/pubmed/23881275 http://dx.doi.org/10.3390/v5071664 |
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