Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus

The secret cave cricket, Ceuthophilus secretes Scudder (Orthroptera: Rhaphidophoridae), is an obligate trogloxene endemic to central Texas, USA, and is a primary source of energy and nutrients for sensitive cave ecosystems. In this study, nine polymorphic microsatellite markers were developed from g...

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Detalles Bibliográficos
Autores principales: Hutchison, Nichole, Leberg, Paul. L., Lance, Richard F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3740920/
https://www.ncbi.nlm.nih.gov/pubmed/23909682
http://dx.doi.org/10.1673/031.013.6401
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author Hutchison, Nichole
Leberg, Paul. L.
Lance, Richard F.
author_facet Hutchison, Nichole
Leberg, Paul. L.
Lance, Richard F.
author_sort Hutchison, Nichole
collection PubMed
description The secret cave cricket, Ceuthophilus secretes Scudder (Orthroptera: Rhaphidophoridae), is an obligate trogloxene endemic to central Texas, USA, and is a primary source of energy and nutrients for sensitive cave ecosystems. In this study, nine polymorphic microsatellite markers were developed from genomic DNA of C. secretes. Genotypes of 120 individuals sampled from four localities on the Fort Hood Military Reservation, Killeen, Texas, were analyzed to characterize the polymorphism at each locus. The number of alleles ranged from 19 to 46. All paired loci were in linkage equilibrium. Four loci had significant deviations from Hardy-Weinberg equilibrium. Observed heterozygosity varied from 0.793 to 0.959. These loci provide a means of characterizing population genetic structure in this species.
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spelling pubmed-37409202013-08-13 Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus Hutchison, Nichole Leberg, Paul. L. Lance, Richard F. J Insect Sci Article The secret cave cricket, Ceuthophilus secretes Scudder (Orthroptera: Rhaphidophoridae), is an obligate trogloxene endemic to central Texas, USA, and is a primary source of energy and nutrients for sensitive cave ecosystems. In this study, nine polymorphic microsatellite markers were developed from genomic DNA of C. secretes. Genotypes of 120 individuals sampled from four localities on the Fort Hood Military Reservation, Killeen, Texas, were analyzed to characterize the polymorphism at each locus. The number of alleles ranged from 19 to 46. All paired loci were in linkage equilibrium. Four loci had significant deviations from Hardy-Weinberg equilibrium. Observed heterozygosity varied from 0.793 to 0.959. These loci provide a means of characterizing population genetic structure in this species. University of Wisconsin Library 2013-06-25 /pmc/articles/PMC3740920/ /pubmed/23909682 http://dx.doi.org/10.1673/031.013.6401 Text en © 2013 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Hutchison, Nichole
Leberg, Paul. L.
Lance, Richard F.
Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus
title Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus
title_full Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus
title_fullStr Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus
title_full_unstemmed Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus
title_short Development of Novel Microsatellite Markers for the Secret Cave Cricket, Ceuthophilus secretus
title_sort development of novel microsatellite markers for the secret cave cricket, ceuthophilus secretus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3740920/
https://www.ncbi.nlm.nih.gov/pubmed/23909682
http://dx.doi.org/10.1673/031.013.6401
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