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Optimisation of Recombinant Production of Active Human Cardiac SERCA2a ATPase

Methods for recombinant production of eukaryotic membrane proteins, yielding sufficient quantity and quality of protein for structural biology, remain a challenge. We describe here, expression and purification optimisation of the human SERCA2a cardiac isoform of Ca(2+) translocating ATPase, using Sa...

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Detalles Bibliográficos
Autores principales:	Antaloae, Ana V., Montigny, Cédric, le Maire, Marc, Watson, Kimberly A., Sørensen, Thomas L.-M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3741278/ https://www.ncbi.nlm.nih.gov/pubmed/23951256 http://dx.doi.org/10.1371/journal.pone.0071842

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