Screening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains

Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast...

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Detalles Bibliográficos
Autores principales: Shi, Shanliang, Ro, Hyeon-Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2010
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3741600/
https://www.ncbi.nlm.nih.gov/pubmed/23956629
http://dx.doi.org/10.4489/MYCO.2010.38.1.070
Descripción
Sumario:Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over 30℃. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from 30℃ to 37℃. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.

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