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Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR
We propose a kinetic model for the activation of the las regulon in the opportunistic pathogen Pseudomonas aeruginosa. The model is based on in vitro data and accounts for the LasR dimerization and consecutive activation by binding of two OdDHL signal molecules. Experimentally, the production of the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742191/ https://www.ncbi.nlm.nih.gov/pubmed/23807499 http://dx.doi.org/10.3390/ijms140713360 |
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author | Claussen, Anetta Jakobsen, Tim Holm Bjarnsholt, Thomas Givskov, Michael Welch, Martin Ferkinghoff-Borg, Jesper Sams, Thomas |
author_facet | Claussen, Anetta Jakobsen, Tim Holm Bjarnsholt, Thomas Givskov, Michael Welch, Martin Ferkinghoff-Borg, Jesper Sams, Thomas |
author_sort | Claussen, Anetta |
collection | PubMed |
description | We propose a kinetic model for the activation of the las regulon in the opportunistic pathogen Pseudomonas aeruginosa. The model is based on in vitro data and accounts for the LasR dimerization and consecutive activation by binding of two OdDHL signal molecules. Experimentally, the production of the active LasR quorum-sensing regulator was studied in an Escherichia coli background as a function of signal molecule concentration. The functional activity of the regulator was monitored via a GFP reporter fusion to lasB expressed from the native lasB promoter. The new data shows that the active form of the LasR dimer binds two signal molecules cooperatively and that the timescale for reaching saturation is independent of the signal molecule concentration. This favors a picture where the dimerized regulator is protected against proteases and remains protected as it is activated through binding of two successive signal molecules. In absence of signal molecules, the dimerized regulator can dissociate and degrade through proteolytic turnover of the monomer. This resolves the apparent contradiction between our data and recent reports that the fully protected dimer is able to “degrade” when the induction of LasR ceases. |
format | Online Article Text |
id | pubmed-3742191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-37421912013-08-13 Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR Claussen, Anetta Jakobsen, Tim Holm Bjarnsholt, Thomas Givskov, Michael Welch, Martin Ferkinghoff-Borg, Jesper Sams, Thomas Int J Mol Sci Article We propose a kinetic model for the activation of the las regulon in the opportunistic pathogen Pseudomonas aeruginosa. The model is based on in vitro data and accounts for the LasR dimerization and consecutive activation by binding of two OdDHL signal molecules. Experimentally, the production of the active LasR quorum-sensing regulator was studied in an Escherichia coli background as a function of signal molecule concentration. The functional activity of the regulator was monitored via a GFP reporter fusion to lasB expressed from the native lasB promoter. The new data shows that the active form of the LasR dimer binds two signal molecules cooperatively and that the timescale for reaching saturation is independent of the signal molecule concentration. This favors a picture where the dimerized regulator is protected against proteases and remains protected as it is activated through binding of two successive signal molecules. In absence of signal molecules, the dimerized regulator can dissociate and degrade through proteolytic turnover of the monomer. This resolves the apparent contradiction between our data and recent reports that the fully protected dimer is able to “degrade” when the induction of LasR ceases. Molecular Diversity Preservation International (MDPI) 2013-06-27 /pmc/articles/PMC3742191/ /pubmed/23807499 http://dx.doi.org/10.3390/ijms140713360 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Claussen, Anetta Jakobsen, Tim Holm Bjarnsholt, Thomas Givskov, Michael Welch, Martin Ferkinghoff-Borg, Jesper Sams, Thomas Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR |
title | Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR |
title_full | Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR |
title_fullStr | Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR |
title_full_unstemmed | Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR |
title_short | Kinetic Model for Signal Binding to the Quorum Sensing Regulator LasR |
title_sort | kinetic model for signal binding to the quorum sensing regulator lasr |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742191/ https://www.ncbi.nlm.nih.gov/pubmed/23807499 http://dx.doi.org/10.3390/ijms140713360 |
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