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The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms

In this work, we studied the antimicrobial properties of a nanocomposite system based on a lactose-substituted chitosan and silver nanoparticles: Chitlac-nAg. Twofold serial dilutions of the colloidal Chitlac-nAg solution were both tested on Streptococcus mitis, Streptococcus mutans, and Streptococc...

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Autores principales: Di Giulio, Mara, Di Bartolomeo, Soraya, Di Campli, Emanuela, Sancilio, Silvia, Marsich, Eleonora, Travan, Andrea, Cataldi, Amelia, Cellini, Luigina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742206/
https://www.ncbi.nlm.nih.gov/pubmed/23812080
http://dx.doi.org/10.3390/ijms140713615
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author Di Giulio, Mara
Di Bartolomeo, Soraya
Di Campli, Emanuela
Sancilio, Silvia
Marsich, Eleonora
Travan, Andrea
Cataldi, Amelia
Cellini, Luigina
author_facet Di Giulio, Mara
Di Bartolomeo, Soraya
Di Campli, Emanuela
Sancilio, Silvia
Marsich, Eleonora
Travan, Andrea
Cataldi, Amelia
Cellini, Luigina
author_sort Di Giulio, Mara
collection PubMed
description In this work, we studied the antimicrobial properties of a nanocomposite system based on a lactose-substituted chitosan and silver nanoparticles: Chitlac-nAg. Twofold serial dilutions of the colloidal Chitlac-nAg solution were both tested on Streptococcus mitis, Streptococcus mutans, and Streptococcus oralis planktonic phase and biofilm growth mode as well as on saliva samples. The minimum inhibitory and bactericidal concentrations of Chitlac-nAg were evaluated together with its effect on sessile cell viability, as well as both on biofilm formation and on preformed biofilm. In respect to the planktonic bacteria, Chitlac-nAg showed an inhibitory/bactericidal effect against all streptococcal strains at 0.1% (v/v), except for S. mitis ATCC 6249 that was inhibited at one step less. On preformed biofilm, Chitlac-nAg at a value of 0.2%, was able to inhibit the bacterial growth on the supernatant phase as well as on the mature biofilm. For S. mitis ATCC 6249, the biofilm inhibitory concentration of Chitlac-nAg was 0.1%. At sub-inhibitory concentrations, the Streptococcal strains adhesion capability on a polystyrene surface showed a general reduction following a concentration-dependent-way; a similar effect was obtained for the metabolic biofilm activity. From these results, Chitlac-nAg seems to be a promising antibacterial and antibiofilm agent able to hinder plaque formation.
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spelling pubmed-37422062013-08-13 The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms Di Giulio, Mara Di Bartolomeo, Soraya Di Campli, Emanuela Sancilio, Silvia Marsich, Eleonora Travan, Andrea Cataldi, Amelia Cellini, Luigina Int J Mol Sci Article In this work, we studied the antimicrobial properties of a nanocomposite system based on a lactose-substituted chitosan and silver nanoparticles: Chitlac-nAg. Twofold serial dilutions of the colloidal Chitlac-nAg solution were both tested on Streptococcus mitis, Streptococcus mutans, and Streptococcus oralis planktonic phase and biofilm growth mode as well as on saliva samples. The minimum inhibitory and bactericidal concentrations of Chitlac-nAg were evaluated together with its effect on sessile cell viability, as well as both on biofilm formation and on preformed biofilm. In respect to the planktonic bacteria, Chitlac-nAg showed an inhibitory/bactericidal effect against all streptococcal strains at 0.1% (v/v), except for S. mitis ATCC 6249 that was inhibited at one step less. On preformed biofilm, Chitlac-nAg at a value of 0.2%, was able to inhibit the bacterial growth on the supernatant phase as well as on the mature biofilm. For S. mitis ATCC 6249, the biofilm inhibitory concentration of Chitlac-nAg was 0.1%. At sub-inhibitory concentrations, the Streptococcal strains adhesion capability on a polystyrene surface showed a general reduction following a concentration-dependent-way; a similar effect was obtained for the metabolic biofilm activity. From these results, Chitlac-nAg seems to be a promising antibacterial and antibiofilm agent able to hinder plaque formation. Molecular Diversity Preservation International (MDPI) 2013-06-28 /pmc/articles/PMC3742206/ /pubmed/23812080 http://dx.doi.org/10.3390/ijms140713615 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Di Giulio, Mara
Di Bartolomeo, Soraya
Di Campli, Emanuela
Sancilio, Silvia
Marsich, Eleonora
Travan, Andrea
Cataldi, Amelia
Cellini, Luigina
The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms
title The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms
title_full The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms
title_fullStr The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms
title_full_unstemmed The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms
title_short The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms
title_sort effect of a silver nanoparticle polysaccharide system on streptococcal and saliva-derived biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742206/
https://www.ncbi.nlm.nih.gov/pubmed/23812080
http://dx.doi.org/10.3390/ijms140713615
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