Cargando…

Changes in the Number of Double-Strand DNA Breaks in Chinese Hamster V79 Cells Exposed to γ-Radiation with Different Dose Rates

A comparative investigation of the induction of double-strand DNA breaks (DSBs) in the Chinese hamster V79 cells by γ-radiation at dose rates of 1, 10 and 400 mGy/min (doses ranged from 0.36 to 4.32 Gy) was performed. The acute radiation exposure at a dose rate of 400 mGy/min resulted in the linear...

Descripción completa

Detalles Bibliográficos
Autores principales: Kotenko, Konstantin V., Bushmanov, Andrey Y., Ozerov, Ivan V., Guryev, Denis V., Anchishkina, Natalya A., Smetanina, Nadezhda M., Arkhangelskaya, Ekaterina Y., Vorobyeva, Natalya Y., Osipov, Andreyan N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742213/
https://www.ncbi.nlm.nih.gov/pubmed/23880845
http://dx.doi.org/10.3390/ijms140713719
_version_ 1782280335141109760
author Kotenko, Konstantin V.
Bushmanov, Andrey Y.
Ozerov, Ivan V.
Guryev, Denis V.
Anchishkina, Natalya A.
Smetanina, Nadezhda M.
Arkhangelskaya, Ekaterina Y.
Vorobyeva, Natalya Y.
Osipov, Andreyan N.
author_facet Kotenko, Konstantin V.
Bushmanov, Andrey Y.
Ozerov, Ivan V.
Guryev, Denis V.
Anchishkina, Natalya A.
Smetanina, Nadezhda M.
Arkhangelskaya, Ekaterina Y.
Vorobyeva, Natalya Y.
Osipov, Andreyan N.
author_sort Kotenko, Konstantin V.
collection PubMed
description A comparative investigation of the induction of double-strand DNA breaks (DSBs) in the Chinese hamster V79 cells by γ-radiation at dose rates of 1, 10 and 400 mGy/min (doses ranged from 0.36 to 4.32 Gy) was performed. The acute radiation exposure at a dose rate of 400 mGy/min resulted in the linear dose-dependent increase of the γ-H2AX foci formation. The dose-response curve for the acute exposure was well described by a linear function y = 1.22 + 19.7x, where “y” is an average number of γ-H2AX foci per a cell and “x” is the absorbed dose (Gy). The dose rate reduction down to 10 mGy/min lead to a decreased number of γ-H2AX foci, as well as to a change of the dose-response relationship. Thus, the foci number up to 1.44 Gy increased and reached the “plateau” area between 1.44 and 4.32 Gy. There was only a slight increase of the γ-H2AX foci number (up to 7) in cells after the protracted exposure (up to 72 h) to ionizing radiation at a dose rate of 1 mGy/min. Similar effects of the varying dose rates were obtained when DNA damage was assessed using the comet assay. In general, our results show that the reduction of the radiation dose rate resulted in a significant decrease of DSBs per cell per an absorbed dose.
format Online
Article
Text
id pubmed-3742213
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-37422132013-08-13 Changes in the Number of Double-Strand DNA Breaks in Chinese Hamster V79 Cells Exposed to γ-Radiation with Different Dose Rates Kotenko, Konstantin V. Bushmanov, Andrey Y. Ozerov, Ivan V. Guryev, Denis V. Anchishkina, Natalya A. Smetanina, Nadezhda M. Arkhangelskaya, Ekaterina Y. Vorobyeva, Natalya Y. Osipov, Andreyan N. Int J Mol Sci Article A comparative investigation of the induction of double-strand DNA breaks (DSBs) in the Chinese hamster V79 cells by γ-radiation at dose rates of 1, 10 and 400 mGy/min (doses ranged from 0.36 to 4.32 Gy) was performed. The acute radiation exposure at a dose rate of 400 mGy/min resulted in the linear dose-dependent increase of the γ-H2AX foci formation. The dose-response curve for the acute exposure was well described by a linear function y = 1.22 + 19.7x, where “y” is an average number of γ-H2AX foci per a cell and “x” is the absorbed dose (Gy). The dose rate reduction down to 10 mGy/min lead to a decreased number of γ-H2AX foci, as well as to a change of the dose-response relationship. Thus, the foci number up to 1.44 Gy increased and reached the “plateau” area between 1.44 and 4.32 Gy. There was only a slight increase of the γ-H2AX foci number (up to 7) in cells after the protracted exposure (up to 72 h) to ionizing radiation at a dose rate of 1 mGy/min. Similar effects of the varying dose rates were obtained when DNA damage was assessed using the comet assay. In general, our results show that the reduction of the radiation dose rate resulted in a significant decrease of DSBs per cell per an absorbed dose. Molecular Diversity Preservation International (MDPI) 2013-07-01 /pmc/articles/PMC3742213/ /pubmed/23880845 http://dx.doi.org/10.3390/ijms140713719 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kotenko, Konstantin V.
Bushmanov, Andrey Y.
Ozerov, Ivan V.
Guryev, Denis V.
Anchishkina, Natalya A.
Smetanina, Nadezhda M.
Arkhangelskaya, Ekaterina Y.
Vorobyeva, Natalya Y.
Osipov, Andreyan N.
Changes in the Number of Double-Strand DNA Breaks in Chinese Hamster V79 Cells Exposed to γ-Radiation with Different Dose Rates
title Changes in the Number of Double-Strand DNA Breaks in Chinese Hamster V79 Cells Exposed to γ-Radiation with Different Dose Rates
title_full Changes in the Number of Double-Strand DNA Breaks in Chinese Hamster V79 Cells Exposed to γ-Radiation with Different Dose Rates
title_fullStr Changes in the Number of Double-Strand DNA Breaks in Chinese Hamster V79 Cells Exposed to γ-Radiation with Different Dose Rates
title_full_unstemmed Changes in the Number of Double-Strand DNA Breaks in Chinese Hamster V79 Cells Exposed to γ-Radiation with Different Dose Rates
title_short Changes in the Number of Double-Strand DNA Breaks in Chinese Hamster V79 Cells Exposed to γ-Radiation with Different Dose Rates
title_sort changes in the number of double-strand dna breaks in chinese hamster v79 cells exposed to γ-radiation with different dose rates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742213/
https://www.ncbi.nlm.nih.gov/pubmed/23880845
http://dx.doi.org/10.3390/ijms140713719
work_keys_str_mv AT kotenkokonstantinv changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates
AT bushmanovandreyy changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates
AT ozerovivanv changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates
AT guryevdenisv changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates
AT anchishkinanatalyaa changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates
AT smetaninanadezhdam changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates
AT arkhangelskayaekaterinay changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates
AT vorobyevanatalyay changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates
AT osipovandreyann changesinthenumberofdoublestranddnabreaksinchinesehamsterv79cellsexposedtogradiationwithdifferentdoserates