MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells

Leucine, a branched chain amino acid, is well known to stimulate protein synthesis in skeletal muscle. However, the role of leucine in myoblast proliferation remains unclear. In this study, we found that leucine could promote proliferation of C2C12 cells. Moreover, expressions of miR-27a and myostat...

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Detalles Bibliográficos
Autores principales: Chen, Xiaoling, Huang, Zhiqing, Chen, Daiwen, Yang, Ting, Liu, Guangmang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742232/
https://www.ncbi.nlm.nih.gov/pubmed/23880856
http://dx.doi.org/10.3390/ijms140714076
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author Chen, Xiaoling
Huang, Zhiqing
Chen, Daiwen
Yang, Ting
Liu, Guangmang
author_facet Chen, Xiaoling
Huang, Zhiqing
Chen, Daiwen
Yang, Ting
Liu, Guangmang
author_sort Chen, Xiaoling
collection PubMed
description Leucine, a branched chain amino acid, is well known to stimulate protein synthesis in skeletal muscle. However, the role of leucine in myoblast proliferation remains unclear. In this study, we found that leucine could promote proliferation of C2C12 cells. Moreover, expressions of miR-27a and myostatin (a bona fide target of miR-27a) were upregulated and downregulated, respectively, following leucine treatment. We also found that miR-27a loss-of-function by transfection of a miR-27a inhibitor suppressed the promotion of myoblast proliferation caused by leucine. Our results suggest that miR-27a is induced by leucine and contributes to leucine-induced proliferation promotion of myoblast.
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spelling pubmed-37422322013-08-13 MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells Chen, Xiaoling Huang, Zhiqing Chen, Daiwen Yang, Ting Liu, Guangmang Int J Mol Sci Article Leucine, a branched chain amino acid, is well known to stimulate protein synthesis in skeletal muscle. However, the role of leucine in myoblast proliferation remains unclear. In this study, we found that leucine could promote proliferation of C2C12 cells. Moreover, expressions of miR-27a and myostatin (a bona fide target of miR-27a) were upregulated and downregulated, respectively, following leucine treatment. We also found that miR-27a loss-of-function by transfection of a miR-27a inhibitor suppressed the promotion of myoblast proliferation caused by leucine. Our results suggest that miR-27a is induced by leucine and contributes to leucine-induced proliferation promotion of myoblast. Molecular Diversity Preservation International (MDPI) 2013-07-08 /pmc/articles/PMC3742232/ /pubmed/23880856 http://dx.doi.org/10.3390/ijms140714076 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Chen, Xiaoling
Huang, Zhiqing
Chen, Daiwen
Yang, Ting
Liu, Guangmang
MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells
title MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells
title_full MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells
title_fullStr MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells
title_full_unstemmed MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells
title_short MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells
title_sort microrna-27a is induced by leucine and contributes to leucine-induced proliferation promotion in c2c12 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742232/
https://www.ncbi.nlm.nih.gov/pubmed/23880856
http://dx.doi.org/10.3390/ijms140714076
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