Cargando…

Fluvastatin Influences Hair Color in C57Bl/6 Mice

Our recent in vitro experiments suggest that fluvastatin may influence tyrosinase (key enzyme of melanogenesis) synthesis. The aim of the present study was to verify those findings in experiments, in vitro, in melanoma cell line, and in vivo, in mice. The expression of tyrosinase in B16F10 melanoma...

Descripción completa

Detalles Bibliográficos
Autores principales: Galus, Ryszard, Włodarski, Krzysztof, Malejczyk, Jacek, JóŸwiak, Jarosław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742247/
https://www.ncbi.nlm.nih.gov/pubmed/23846727
http://dx.doi.org/10.3390/ijms140714333
_version_ 1782280342965583872
author Galus, Ryszard
Włodarski, Krzysztof
Malejczyk, Jacek
JóŸwiak, Jarosław
author_facet Galus, Ryszard
Włodarski, Krzysztof
Malejczyk, Jacek
JóŸwiak, Jarosław
author_sort Galus, Ryszard
collection PubMed
description Our recent in vitro experiments suggest that fluvastatin may influence tyrosinase (key enzyme of melanogenesis) synthesis. The aim of the present study was to verify those findings in experiments, in vitro, in melanoma cell line, and in vivo, in mice. The expression of tyrosinase in B16F10 melanoma cell line, after induction of melanogenesis by UVB irradiation, was examined by Western blot analysis. Afterwards, the effect of fluvastatin on melanin synthesis in hair follicles of C57Bl/6 mice was investigated. The expression of tyrosinase was reduced in the presence of fluvastatin. In mice after anagen induction over the dorsal skin, gel containing fluvastatin in various concentrations was injected subcutaneously, while in part of control groups of mice, gel with placebo was injected. In addition, gel with fluvastatin was injected to four week-old mice (mice in first postnatal anagen) without anagen induction. In extension, injections of gel with fluvastatin or placebo were performed in mice without anagen induction (but after first postnatal anagen). In part of study group of mice (mice after anagen induction and injection of fluvastatin) regrowth of depigmented hair was observed, while in all control groups (mice after injection of placebo), such hair depigmentation over the skin area was not found. In conclusion, this study, for the first time, shows that fluvastatin might affect melanin synthesis in vivo.
format Online
Article
Text
id pubmed-3742247
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-37422472013-08-13 Fluvastatin Influences Hair Color in C57Bl/6 Mice Galus, Ryszard Włodarski, Krzysztof Malejczyk, Jacek JóŸwiak, Jarosław Int J Mol Sci Article Our recent in vitro experiments suggest that fluvastatin may influence tyrosinase (key enzyme of melanogenesis) synthesis. The aim of the present study was to verify those findings in experiments, in vitro, in melanoma cell line, and in vivo, in mice. The expression of tyrosinase in B16F10 melanoma cell line, after induction of melanogenesis by UVB irradiation, was examined by Western blot analysis. Afterwards, the effect of fluvastatin on melanin synthesis in hair follicles of C57Bl/6 mice was investigated. The expression of tyrosinase was reduced in the presence of fluvastatin. In mice after anagen induction over the dorsal skin, gel containing fluvastatin in various concentrations was injected subcutaneously, while in part of control groups of mice, gel with placebo was injected. In addition, gel with fluvastatin was injected to four week-old mice (mice in first postnatal anagen) without anagen induction. In extension, injections of gel with fluvastatin or placebo were performed in mice without anagen induction (but after first postnatal anagen). In part of study group of mice (mice after anagen induction and injection of fluvastatin) regrowth of depigmented hair was observed, while in all control groups (mice after injection of placebo), such hair depigmentation over the skin area was not found. In conclusion, this study, for the first time, shows that fluvastatin might affect melanin synthesis in vivo. Molecular Diversity Preservation International (MDPI) 2013-07-10 /pmc/articles/PMC3742247/ /pubmed/23846727 http://dx.doi.org/10.3390/ijms140714333 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Galus, Ryszard
Włodarski, Krzysztof
Malejczyk, Jacek
JóŸwiak, Jarosław
Fluvastatin Influences Hair Color in C57Bl/6 Mice
title Fluvastatin Influences Hair Color in C57Bl/6 Mice
title_full Fluvastatin Influences Hair Color in C57Bl/6 Mice
title_fullStr Fluvastatin Influences Hair Color in C57Bl/6 Mice
title_full_unstemmed Fluvastatin Influences Hair Color in C57Bl/6 Mice
title_short Fluvastatin Influences Hair Color in C57Bl/6 Mice
title_sort fluvastatin influences hair color in c57bl/6 mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742247/
https://www.ncbi.nlm.nih.gov/pubmed/23846727
http://dx.doi.org/10.3390/ijms140714333
work_keys_str_mv AT galusryszard fluvastatininfluenceshaircolorinc57bl6mice
AT włodarskikrzysztof fluvastatininfluenceshaircolorinc57bl6mice
AT malejczykjacek fluvastatininfluenceshaircolorinc57bl6mice
AT joywiakjarosław fluvastatininfluenceshaircolorinc57bl6mice