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Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level
Biotechnological approaches using genetic modifications such as homologous gene overexpression can be used to decode gene functions under well-defined circumstances. However, only the recording of the resulting phenotypes allows inferences about the impact of the modification on the organisms’ evolu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742292/ https://www.ncbi.nlm.nih.gov/pubmed/23880863 http://dx.doi.org/10.3390/ijms140715141 |
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author | Vaas, Lea A. I. Marheine, Maja Sikorski, Johannes Göker, Markus Schumacher, Heinz-Martin |
author_facet | Vaas, Lea A. I. Marheine, Maja Sikorski, Johannes Göker, Markus Schumacher, Heinz-Martin |
author_sort | Vaas, Lea A. I. |
collection | PubMed |
description | Biotechnological approaches using genetic modifications such as homologous gene overexpression can be used to decode gene functions under well-defined circumstances. However, only the recording of the resulting phenotypes allows inferences about the impact of the modification on the organisms’ evolutionary, ecological or economic performance. We here compare a potato wild-type cell line with two genetically engineered cell cultures homologously overexpressing Pathogenesis Related Protein 10a (pr-10a). A detailed analysis of the relative gene-expression patterns of pr-10a and its regulators sebf and pti4 over time provides insights into the molecular response of heterotrophic cells to distinct osmotic and salt-stress conditions. Furthermore, this system serves as an exemplar for the tracing of respiration kinetics as a faster and more sensitive alternative to the laborious and time-consuming recording of growth curves. The utility and characteristics of the resulting data type and the requirements for its appropriate analysis are figured out. It is demonstrated how this novel type of phenotypic information together with the gene-expression-data provides valuable insights into the effect of genetic modifications on the behaviour of cells on both the molecular and the macroscopic level. |
format | Online Article Text |
id | pubmed-3742292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-37422922013-08-13 Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level Vaas, Lea A. I. Marheine, Maja Sikorski, Johannes Göker, Markus Schumacher, Heinz-Martin Int J Mol Sci Article Biotechnological approaches using genetic modifications such as homologous gene overexpression can be used to decode gene functions under well-defined circumstances. However, only the recording of the resulting phenotypes allows inferences about the impact of the modification on the organisms’ evolutionary, ecological or economic performance. We here compare a potato wild-type cell line with two genetically engineered cell cultures homologously overexpressing Pathogenesis Related Protein 10a (pr-10a). A detailed analysis of the relative gene-expression patterns of pr-10a and its regulators sebf and pti4 over time provides insights into the molecular response of heterotrophic cells to distinct osmotic and salt-stress conditions. Furthermore, this system serves as an exemplar for the tracing of respiration kinetics as a faster and more sensitive alternative to the laborious and time-consuming recording of growth curves. The utility and characteristics of the resulting data type and the requirements for its appropriate analysis are figured out. It is demonstrated how this novel type of phenotypic information together with the gene-expression-data provides valuable insights into the effect of genetic modifications on the behaviour of cells on both the molecular and the macroscopic level. Molecular Diversity Preservation International (MDPI) 2013-07-22 /pmc/articles/PMC3742292/ /pubmed/23880863 http://dx.doi.org/10.3390/ijms140715141 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Vaas, Lea A. I. Marheine, Maja Sikorski, Johannes Göker, Markus Schumacher, Heinz-Martin Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level |
title | Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level |
title_full | Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level |
title_fullStr | Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level |
title_full_unstemmed | Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level |
title_short | Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level |
title_sort | impacts of pr-10a overexpression at the molecular and the phenotypic level |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742292/ https://www.ncbi.nlm.nih.gov/pubmed/23880863 http://dx.doi.org/10.3390/ijms140715141 |
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