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Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells

Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transpla...

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Autores principales: Makarem, Maisam, Kannan, Nagarajan, Nguyen, Long V., Knapp, David J. H. F., Balani, Sneha, Prater, Michael D., Stingl, John, Raouf, Afshin, Nemirovsky, Oksana, Eirew, Peter, Eaves, Connie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742452/
https://www.ncbi.nlm.nih.gov/pubmed/23966837
http://dx.doi.org/10.1371/journal.pbio.1001630
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author Makarem, Maisam
Kannan, Nagarajan
Nguyen, Long V.
Knapp, David J. H. F.
Balani, Sneha
Prater, Michael D.
Stingl, John
Raouf, Afshin
Nemirovsky, Oksana
Eirew, Peter
Eaves, Connie J.
author_facet Makarem, Maisam
Kannan, Nagarajan
Nguyen, Long V.
Knapp, David J. H. F.
Balani, Sneha
Prater, Michael D.
Stingl, John
Raouf, Afshin
Nemirovsky, Oksana
Eirew, Peter
Eaves, Connie J.
author_sort Makarem, Maisam
collection PubMed
description Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transplantable regenerative activity are first detectable a few days before birth. They share some phenotypic properties with their adult counterparts but are enriched in a subpopulation that displays a distinct gene expression profile. Here we show that fetal mammary epithelial cells have a greater direct and inducible growth potential than their adult counterparts. The latter feature is revealed in a novel culture system that enables large numbers of in vitro clonogenic progenitors as well as mammary stem cells with serially transplantable activity to be produced within 7 days from single fetal or adult input cells. We further show that these responses are highly dependent on novel factors produced by fibroblasts. These findings provide new avenues for elucidating mechanisms that regulate normal mammary epithelial stem cell properties at the single-cell level, how these change during development, and how their perturbation may contribute to transformation.
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spelling pubmed-37424522013-08-21 Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells Makarem, Maisam Kannan, Nagarajan Nguyen, Long V. Knapp, David J. H. F. Balani, Sneha Prater, Michael D. Stingl, John Raouf, Afshin Nemirovsky, Oksana Eirew, Peter Eaves, Connie J. PLoS Biol Research Article Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transplantable regenerative activity are first detectable a few days before birth. They share some phenotypic properties with their adult counterparts but are enriched in a subpopulation that displays a distinct gene expression profile. Here we show that fetal mammary epithelial cells have a greater direct and inducible growth potential than their adult counterparts. The latter feature is revealed in a novel culture system that enables large numbers of in vitro clonogenic progenitors as well as mammary stem cells with serially transplantable activity to be produced within 7 days from single fetal or adult input cells. We further show that these responses are highly dependent on novel factors produced by fibroblasts. These findings provide new avenues for elucidating mechanisms that regulate normal mammary epithelial stem cell properties at the single-cell level, how these change during development, and how their perturbation may contribute to transformation. Public Library of Science 2013-08-13 /pmc/articles/PMC3742452/ /pubmed/23966837 http://dx.doi.org/10.1371/journal.pbio.1001630 Text en © 2013 Makarem et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Makarem, Maisam
Kannan, Nagarajan
Nguyen, Long V.
Knapp, David J. H. F.
Balani, Sneha
Prater, Michael D.
Stingl, John
Raouf, Afshin
Nemirovsky, Oksana
Eirew, Peter
Eaves, Connie J.
Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells
title Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells
title_full Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells
title_fullStr Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells
title_full_unstemmed Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells
title_short Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells
title_sort developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742452/
https://www.ncbi.nlm.nih.gov/pubmed/23966837
http://dx.doi.org/10.1371/journal.pbio.1001630
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