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Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells
Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transpla...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742452/ https://www.ncbi.nlm.nih.gov/pubmed/23966837 http://dx.doi.org/10.1371/journal.pbio.1001630 |
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author | Makarem, Maisam Kannan, Nagarajan Nguyen, Long V. Knapp, David J. H. F. Balani, Sneha Prater, Michael D. Stingl, John Raouf, Afshin Nemirovsky, Oksana Eirew, Peter Eaves, Connie J. |
author_facet | Makarem, Maisam Kannan, Nagarajan Nguyen, Long V. Knapp, David J. H. F. Balani, Sneha Prater, Michael D. Stingl, John Raouf, Afshin Nemirovsky, Oksana Eirew, Peter Eaves, Connie J. |
author_sort | Makarem, Maisam |
collection | PubMed |
description | Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transplantable regenerative activity are first detectable a few days before birth. They share some phenotypic properties with their adult counterparts but are enriched in a subpopulation that displays a distinct gene expression profile. Here we show that fetal mammary epithelial cells have a greater direct and inducible growth potential than their adult counterparts. The latter feature is revealed in a novel culture system that enables large numbers of in vitro clonogenic progenitors as well as mammary stem cells with serially transplantable activity to be produced within 7 days from single fetal or adult input cells. We further show that these responses are highly dependent on novel factors produced by fibroblasts. These findings provide new avenues for elucidating mechanisms that regulate normal mammary epithelial stem cell properties at the single-cell level, how these change during development, and how their perturbation may contribute to transformation. |
format | Online Article Text |
id | pubmed-3742452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37424522013-08-21 Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells Makarem, Maisam Kannan, Nagarajan Nguyen, Long V. Knapp, David J. H. F. Balani, Sneha Prater, Michael D. Stingl, John Raouf, Afshin Nemirovsky, Oksana Eirew, Peter Eaves, Connie J. PLoS Biol Research Article Many normal adult tissues contain rare stem cells with extensive self-maintaining regenerative potential. During development, the stem cells of the hematopoietic and neural systems undergo intrinsically specified changes in their self-renewal potential. In the mouse, mammary stem cells with transplantable regenerative activity are first detectable a few days before birth. They share some phenotypic properties with their adult counterparts but are enriched in a subpopulation that displays a distinct gene expression profile. Here we show that fetal mammary epithelial cells have a greater direct and inducible growth potential than their adult counterparts. The latter feature is revealed in a novel culture system that enables large numbers of in vitro clonogenic progenitors as well as mammary stem cells with serially transplantable activity to be produced within 7 days from single fetal or adult input cells. We further show that these responses are highly dependent on novel factors produced by fibroblasts. These findings provide new avenues for elucidating mechanisms that regulate normal mammary epithelial stem cell properties at the single-cell level, how these change during development, and how their perturbation may contribute to transformation. Public Library of Science 2013-08-13 /pmc/articles/PMC3742452/ /pubmed/23966837 http://dx.doi.org/10.1371/journal.pbio.1001630 Text en © 2013 Makarem et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Makarem, Maisam Kannan, Nagarajan Nguyen, Long V. Knapp, David J. H. F. Balani, Sneha Prater, Michael D. Stingl, John Raouf, Afshin Nemirovsky, Oksana Eirew, Peter Eaves, Connie J. Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells |
title | Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells |
title_full | Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells |
title_fullStr | Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells |
title_full_unstemmed | Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells |
title_short | Developmental Changes in the in Vitro Activated Regenerative Activity of Primitive Mammary Epithelial Cells |
title_sort | developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742452/ https://www.ncbi.nlm.nih.gov/pubmed/23966837 http://dx.doi.org/10.1371/journal.pbio.1001630 |
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