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Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents

BACKGROUND: DNA aptamers generated by cell-SELEX offer an attractive alternative to antibodies, but generating aptamers to specific, known membrane protein targets has proven challenging, and has severely limited the use of aptamers as affinity reagents for cell identification and purification. METH...

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Detalles Bibliográficos
Autores principales: Meyer, Susanne, Maufort, John P., Nie, Jeff, Stewart, Ron, McIntosh, Brian E., Conti, Lisa R., Ahmad, Kareem M., Soh, H. Tom, Thomson, James A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742456/
https://www.ncbi.nlm.nih.gov/pubmed/23967247
http://dx.doi.org/10.1371/journal.pone.0071798

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