A Tet-On System for DRD1-Expressing Cells

Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of t...

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Detalles Bibliográficos
Autores principales: Yao, Fayi, Walker, Paul D., MacKenzie, Robert G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742517/
https://www.ncbi.nlm.nih.gov/pubmed/23967330
http://dx.doi.org/10.1371/journal.pone.0072681
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author Yao, Fayi
Walker, Paul D.
MacKenzie, Robert G.
author_facet Yao, Fayi
Walker, Paul D.
MacKenzie, Robert G.
author_sort Yao, Fayi
collection PubMed
description Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of tet-operated transgenes specifically within DRD1-expressing cells of transgenic mice. It is shown that the DRD1-rtTA BAC-driven expression of a tet-operated reporter is under tight regulation by doxycycline and is restricted to DRD1-expressing brain regions. The model will be a useful research tool in studies of movement and reward and associated pathologies such as Parkinson’s disease and addiction.
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spelling pubmed-37425172013-08-21 A Tet-On System for DRD1-Expressing Cells Yao, Fayi Walker, Paul D. MacKenzie, Robert G. PLoS One Research Article Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of tet-operated transgenes specifically within DRD1-expressing cells of transgenic mice. It is shown that the DRD1-rtTA BAC-driven expression of a tet-operated reporter is under tight regulation by doxycycline and is restricted to DRD1-expressing brain regions. The model will be a useful research tool in studies of movement and reward and associated pathologies such as Parkinson’s disease and addiction. Public Library of Science 2013-08-13 /pmc/articles/PMC3742517/ /pubmed/23967330 http://dx.doi.org/10.1371/journal.pone.0072681 Text en © 2013 Yao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yao, Fayi
Walker, Paul D.
MacKenzie, Robert G.
A Tet-On System for DRD1-Expressing Cells
title A Tet-On System for DRD1-Expressing Cells
title_full A Tet-On System for DRD1-Expressing Cells
title_fullStr A Tet-On System for DRD1-Expressing Cells
title_full_unstemmed A Tet-On System for DRD1-Expressing Cells
title_short A Tet-On System for DRD1-Expressing Cells
title_sort tet-on system for drd1-expressing cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742517/
https://www.ncbi.nlm.nih.gov/pubmed/23967330
http://dx.doi.org/10.1371/journal.pone.0072681
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