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Genetic Variants on 3q21 and in the Sp8 Transcription Factor Gene (SP8) as Susceptibility Loci for Psychotic Disorders: A Genetic Association Study

BACKGROUND: Recent genome-wide association studies (GWASs) investigating bipolar disorder (BD) have detected a number of susceptibility genes. These studies have also provided novel insight into shared genetic components between BD and schizophrenia (SCZ), two major psychotic disorders. To examine t...

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Autores principales: Kondo, Kenji, Ikeda, Masashi, Kajio, Yusuke, Saito, Takeo, Iwayama, Yoshimi, Aleksic, Branko, Yamada, Kazuo, Toyota, Tomoko, Hattori, Eiji, Ujike, Hiroshi, Inada, Toshiya, Kunugi, Hiroshi, Kato, Tadafumi, Yoshikawa, Takeo, Ozaki, Norio, Iwata, Nakao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742587/
https://www.ncbi.nlm.nih.gov/pubmed/23967141
http://dx.doi.org/10.1371/journal.pone.0070964
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author Kondo, Kenji
Ikeda, Masashi
Kajio, Yusuke
Saito, Takeo
Iwayama, Yoshimi
Aleksic, Branko
Yamada, Kazuo
Toyota, Tomoko
Hattori, Eiji
Ujike, Hiroshi
Inada, Toshiya
Kunugi, Hiroshi
Kato, Tadafumi
Yoshikawa, Takeo
Ozaki, Norio
Iwata, Nakao
author_facet Kondo, Kenji
Ikeda, Masashi
Kajio, Yusuke
Saito, Takeo
Iwayama, Yoshimi
Aleksic, Branko
Yamada, Kazuo
Toyota, Tomoko
Hattori, Eiji
Ujike, Hiroshi
Inada, Toshiya
Kunugi, Hiroshi
Kato, Tadafumi
Yoshikawa, Takeo
Ozaki, Norio
Iwata, Nakao
author_sort Kondo, Kenji
collection PubMed
description BACKGROUND: Recent genome-wide association studies (GWASs) investigating bipolar disorder (BD) have detected a number of susceptibility genes. These studies have also provided novel insight into shared genetic components between BD and schizophrenia (SCZ), two major psychotic disorders. To examine the replication of the risk variants for BD and the pleiotropic effect of the variants associated with BD, we conducted a genetic association study of single nucleotide polymorphisms (SNPs) that were selected based upon previous BD GWASs, which targeted psychotic disorders (BD and SCZ) in the Japanese population. METHODS: Forty-eight SNPs were selected based upon previous GWASs. A two-stage analysis was conducted using first-set screening (for all SNPs: BD = 1,012, SCZ = 1,032 and control = 993) and second-set replication samples (for significant SNPs in the screening analysis: BD = 821, SCZ = 1,808 and control = 2,149). We assessed allelic association between BD, SCZ, psychosis (BD+SCZ) and the SNPs selected for the analysis. RESULTS: Eight SNPs revealed nominal association signals for all comparisons (P(uncorrected)<0.05). Among these SNPs, the top two SNPs (associated with psychosis: P(corrected) = 0.048 and 0.037 for rs2251219 and rs2709722, respectively) were further assessed in the second-set samples, and we replicated the signals from the initial screening analysis (associated with psychosis: P(corrected) = 0.0070 and 0.033 for rs2251219 and rs2709722, respectively). The meta-analysis between the current and previous GWAS results showed that rs2251219 in Polybromo1 (PBRM1) was significant on genome-wide association level (P = 5×10(−8)) only for BD (P = 9.4×10(−9)) and psychosis (P = 2.0×10(−10)). Although the association of rs2709722 in Sp8 transcription factor (SP8) was suggestive in the Asian population (P = 2.1×10(−7) for psychosis), this signal weakened when the samples size was increased by including data from a Caucasian population (P = 4.3×10(−3)). CONCLUSIONS: We found 3p21.1 (including PBRM1, strong linkage disequilibrium made it difficult to pinpoint the risk genes) and SP8 as risk loci for BD, SCZ and psychosis. Further replication studies will be required for conclusive results.
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spelling pubmed-37425872013-08-21 Genetic Variants on 3q21 and in the Sp8 Transcription Factor Gene (SP8) as Susceptibility Loci for Psychotic Disorders: A Genetic Association Study Kondo, Kenji Ikeda, Masashi Kajio, Yusuke Saito, Takeo Iwayama, Yoshimi Aleksic, Branko Yamada, Kazuo Toyota, Tomoko Hattori, Eiji Ujike, Hiroshi Inada, Toshiya Kunugi, Hiroshi Kato, Tadafumi Yoshikawa, Takeo Ozaki, Norio Iwata, Nakao PLoS One Research Article BACKGROUND: Recent genome-wide association studies (GWASs) investigating bipolar disorder (BD) have detected a number of susceptibility genes. These studies have also provided novel insight into shared genetic components between BD and schizophrenia (SCZ), two major psychotic disorders. To examine the replication of the risk variants for BD and the pleiotropic effect of the variants associated with BD, we conducted a genetic association study of single nucleotide polymorphisms (SNPs) that were selected based upon previous BD GWASs, which targeted psychotic disorders (BD and SCZ) in the Japanese population. METHODS: Forty-eight SNPs were selected based upon previous GWASs. A two-stage analysis was conducted using first-set screening (for all SNPs: BD = 1,012, SCZ = 1,032 and control = 993) and second-set replication samples (for significant SNPs in the screening analysis: BD = 821, SCZ = 1,808 and control = 2,149). We assessed allelic association between BD, SCZ, psychosis (BD+SCZ) and the SNPs selected for the analysis. RESULTS: Eight SNPs revealed nominal association signals for all comparisons (P(uncorrected)<0.05). Among these SNPs, the top two SNPs (associated with psychosis: P(corrected) = 0.048 and 0.037 for rs2251219 and rs2709722, respectively) were further assessed in the second-set samples, and we replicated the signals from the initial screening analysis (associated with psychosis: P(corrected) = 0.0070 and 0.033 for rs2251219 and rs2709722, respectively). The meta-analysis between the current and previous GWAS results showed that rs2251219 in Polybromo1 (PBRM1) was significant on genome-wide association level (P = 5×10(−8)) only for BD (P = 9.4×10(−9)) and psychosis (P = 2.0×10(−10)). Although the association of rs2709722 in Sp8 transcription factor (SP8) was suggestive in the Asian population (P = 2.1×10(−7) for psychosis), this signal weakened when the samples size was increased by including data from a Caucasian population (P = 4.3×10(−3)). CONCLUSIONS: We found 3p21.1 (including PBRM1, strong linkage disequilibrium made it difficult to pinpoint the risk genes) and SP8 as risk loci for BD, SCZ and psychosis. Further replication studies will be required for conclusive results. Public Library of Science 2013-08-13 /pmc/articles/PMC3742587/ /pubmed/23967141 http://dx.doi.org/10.1371/journal.pone.0070964 Text en © 2013 Kondo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kondo, Kenji
Ikeda, Masashi
Kajio, Yusuke
Saito, Takeo
Iwayama, Yoshimi
Aleksic, Branko
Yamada, Kazuo
Toyota, Tomoko
Hattori, Eiji
Ujike, Hiroshi
Inada, Toshiya
Kunugi, Hiroshi
Kato, Tadafumi
Yoshikawa, Takeo
Ozaki, Norio
Iwata, Nakao
Genetic Variants on 3q21 and in the Sp8 Transcription Factor Gene (SP8) as Susceptibility Loci for Psychotic Disorders: A Genetic Association Study
title Genetic Variants on 3q21 and in the Sp8 Transcription Factor Gene (SP8) as Susceptibility Loci for Psychotic Disorders: A Genetic Association Study
title_full Genetic Variants on 3q21 and in the Sp8 Transcription Factor Gene (SP8) as Susceptibility Loci for Psychotic Disorders: A Genetic Association Study
title_fullStr Genetic Variants on 3q21 and in the Sp8 Transcription Factor Gene (SP8) as Susceptibility Loci for Psychotic Disorders: A Genetic Association Study
title_full_unstemmed Genetic Variants on 3q21 and in the Sp8 Transcription Factor Gene (SP8) as Susceptibility Loci for Psychotic Disorders: A Genetic Association Study
title_short Genetic Variants on 3q21 and in the Sp8 Transcription Factor Gene (SP8) as Susceptibility Loci for Psychotic Disorders: A Genetic Association Study
title_sort genetic variants on 3q21 and in the sp8 transcription factor gene (sp8) as susceptibility loci for psychotic disorders: a genetic association study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742587/
https://www.ncbi.nlm.nih.gov/pubmed/23967141
http://dx.doi.org/10.1371/journal.pone.0070964
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