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Pitfalls encountered while investigating genetic elements by PCR
The unprecedented wealth of databases that have become available in the era of next-generation sequencing has considerably increased our knowledge of bacterial genetic elements (GEs). At the same time, the advent of single-cell based approaches has brought realization that unsuspected heterogeneity...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742598/ https://www.ncbi.nlm.nih.gov/pubmed/23956950 http://dx.doi.org/10.4161/mge.25255 |
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author | Palmieri, Claudio Brenciani, Andrea Mingoia, Marina Giovanetti, Eleonora Facinelli, Bruna Varaldo, Pietro E. |
author_facet | Palmieri, Claudio Brenciani, Andrea Mingoia, Marina Giovanetti, Eleonora Facinelli, Bruna Varaldo, Pietro E. |
author_sort | Palmieri, Claudio |
collection | PubMed |
description | The unprecedented wealth of databases that have become available in the era of next-generation sequencing has considerably increased our knowledge of bacterial genetic elements (GEs). At the same time, the advent of single-cell based approaches has brought realization that unsuspected heterogeneity may occur in the bacterial population from a single colony. The increasing use of PCR-based techniques to study new GEs requires careful consideration of the possible different PCR targets associated with different subpopulations if incorrect or incomplete interpretations are to be avoided. In this commentary, confining ourselves to our direct experience, we illustrate some examples of PCR pitfalls that may be encountered while investigating GEs. |
format | Online Article Text |
id | pubmed-3742598 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-37425982013-08-16 Pitfalls encountered while investigating genetic elements by PCR Palmieri, Claudio Brenciani, Andrea Mingoia, Marina Giovanetti, Eleonora Facinelli, Bruna Varaldo, Pietro E. Mob Genet Elements Commentary The unprecedented wealth of databases that have become available in the era of next-generation sequencing has considerably increased our knowledge of bacterial genetic elements (GEs). At the same time, the advent of single-cell based approaches has brought realization that unsuspected heterogeneity may occur in the bacterial population from a single colony. The increasing use of PCR-based techniques to study new GEs requires careful consideration of the possible different PCR targets associated with different subpopulations if incorrect or incomplete interpretations are to be avoided. In this commentary, confining ourselves to our direct experience, we illustrate some examples of PCR pitfalls that may be encountered while investigating GEs. Landes Bioscience 2013-05-01 2013-06-03 /pmc/articles/PMC3742598/ /pubmed/23956950 http://dx.doi.org/10.4161/mge.25255 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Commentary Palmieri, Claudio Brenciani, Andrea Mingoia, Marina Giovanetti, Eleonora Facinelli, Bruna Varaldo, Pietro E. Pitfalls encountered while investigating genetic elements by PCR |
title | Pitfalls encountered while investigating genetic elements by PCR |
title_full | Pitfalls encountered while investigating genetic elements by PCR |
title_fullStr | Pitfalls encountered while investigating genetic elements by PCR |
title_full_unstemmed | Pitfalls encountered while investigating genetic elements by PCR |
title_short | Pitfalls encountered while investigating genetic elements by PCR |
title_sort | pitfalls encountered while investigating genetic elements by pcr |
topic | Commentary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742598/ https://www.ncbi.nlm.nih.gov/pubmed/23956950 http://dx.doi.org/10.4161/mge.25255 |
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