Cargando…

Downregulation of L-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells

Gastric cancer is the second leading cause of cancer-related mortality worldwide. Identifying the molecules that play critical roles in the development of gastric cancer, and clarifying their mechanisms, will contribute to the development of novel molecularly targeted therapeutic drugs. Recently, th...

Descripción completa

Detalles Bibliográficos
Autores principales: SHI, LIANGHUI, LUO, WENPING, HUANG, WENBING, HUANG, SHISUI, HUANG, GUANGYAN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742819/
https://www.ncbi.nlm.nih.gov/pubmed/23946786
http://dx.doi.org/10.3892/ol.2013.1342
_version_ 1782280419771678720
author SHI, LIANGHUI
LUO, WENPING
HUANG, WENBING
HUANG, SHISUI
HUANG, GUANGYAN
author_facet SHI, LIANGHUI
LUO, WENPING
HUANG, WENBING
HUANG, SHISUI
HUANG, GUANGYAN
author_sort SHI, LIANGHUI
collection PubMed
description Gastric cancer is the second leading cause of cancer-related mortality worldwide. Identifying the molecules that play critical roles in the development of gastric cancer, and clarifying their mechanisms, will contribute to the development of novel molecularly targeted therapeutic drugs. Recently, the large (L)-type amino acid transporter 1 (LAT1), a glycoprotein that transports amino acids through the cell membrane when associated with CD98hc, has been demonstrated to be overexpressed in various types of cancer, and to regulate multiple biological process, including cell growth, migration and invasion. However, the involvement of LAT1 in gastric cancer remains unclear. In the present study, stable gastric cancer cell lines with a LAT1 knockdown were established by transfection of constructs with inserted short (sh) RNAs, in order to clarify the role of LAT1 in gastric caner. A significant decrease in LAT1 expression was observed in the established LAT1-silenced SGC7901 cells compared with the corresponding control cells; however, the expression levels of its partner, CD98hc, were not altered. Furthermore, downregulation of LAT1 expression inhibited the proliferation, migration and invasion of gastric cancer cells. In addition, the decreased expression of LAT1 induced cell cycle arrest in the G(1)/M phase. These findings suggested that LAT1 may be significant in the progression and metastasis of gastric cancer, and may be developed as a therapeutic target for cancer therapy.
format Online
Article
Text
id pubmed-3742819
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-37428192013-08-14 Downregulation of L-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells SHI, LIANGHUI LUO, WENPING HUANG, WENBING HUANG, SHISUI HUANG, GUANGYAN Oncol Lett Articles Gastric cancer is the second leading cause of cancer-related mortality worldwide. Identifying the molecules that play critical roles in the development of gastric cancer, and clarifying their mechanisms, will contribute to the development of novel molecularly targeted therapeutic drugs. Recently, the large (L)-type amino acid transporter 1 (LAT1), a glycoprotein that transports amino acids through the cell membrane when associated with CD98hc, has been demonstrated to be overexpressed in various types of cancer, and to regulate multiple biological process, including cell growth, migration and invasion. However, the involvement of LAT1 in gastric cancer remains unclear. In the present study, stable gastric cancer cell lines with a LAT1 knockdown were established by transfection of constructs with inserted short (sh) RNAs, in order to clarify the role of LAT1 in gastric caner. A significant decrease in LAT1 expression was observed in the established LAT1-silenced SGC7901 cells compared with the corresponding control cells; however, the expression levels of its partner, CD98hc, were not altered. Furthermore, downregulation of LAT1 expression inhibited the proliferation, migration and invasion of gastric cancer cells. In addition, the decreased expression of LAT1 induced cell cycle arrest in the G(1)/M phase. These findings suggested that LAT1 may be significant in the progression and metastasis of gastric cancer, and may be developed as a therapeutic target for cancer therapy. D.A. Spandidos 2013-07 2013-05-08 /pmc/articles/PMC3742819/ /pubmed/23946786 http://dx.doi.org/10.3892/ol.2013.1342 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
SHI, LIANGHUI
LUO, WENPING
HUANG, WENBING
HUANG, SHISUI
HUANG, GUANGYAN
Downregulation of L-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells
title Downregulation of L-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells
title_full Downregulation of L-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells
title_fullStr Downregulation of L-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells
title_full_unstemmed Downregulation of L-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells
title_short Downregulation of L-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells
title_sort downregulation of l-type amino acid transporter 1 expression inhibits the growth, migration and invasion of gastric cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742819/
https://www.ncbi.nlm.nih.gov/pubmed/23946786
http://dx.doi.org/10.3892/ol.2013.1342
work_keys_str_mv AT shilianghui downregulationofltypeaminoacidtransporter1expressioninhibitsthegrowthmigrationandinvasionofgastriccancercells
AT luowenping downregulationofltypeaminoacidtransporter1expressioninhibitsthegrowthmigrationandinvasionofgastriccancercells
AT huangwenbing downregulationofltypeaminoacidtransporter1expressioninhibitsthegrowthmigrationandinvasionofgastriccancercells
AT huangshisui downregulationofltypeaminoacidtransporter1expressioninhibitsthegrowthmigrationandinvasionofgastriccancercells
AT huangguangyan downregulationofltypeaminoacidtransporter1expressioninhibitsthegrowthmigrationandinvasionofgastriccancercells