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Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells
Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) provides an alternative to using embryonic stem cells (ESCs). Mesenchymal stem cells derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking of human hairs. Whether these hHF-MSCs...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742959/ https://www.ncbi.nlm.nih.gov/pubmed/23242965 http://dx.doi.org/10.1007/s12015-012-9420-5 |
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author | Wang, Yimei Liu, Jinyu Tan, Xiaohua Li, Gaofeng Gao, Yunhe Liu, Xuejuan Zhang, Lihong Li, Yulin |
author_facet | Wang, Yimei Liu, Jinyu Tan, Xiaohua Li, Gaofeng Gao, Yunhe Liu, Xuejuan Zhang, Lihong Li, Yulin |
author_sort | Wang, Yimei |
collection | PubMed |
description | Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) provides an alternative to using embryonic stem cells (ESCs). Mesenchymal stem cells derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking of human hairs. Whether these hHF-MSCs can be reprogrammed has not been previously reported. Here we report the generation of iPSCs from hHF-MSCs obtained by plucking several hairs. hHF-MSCs were isolated from hair follicle tissues and their mesenchymal nature confirmed by detecting cell surface antigens and multilineage differentiation potential towards adipocytes and osteoblasts. They were then reprogrammed into iPSCs by lentiviral transduction with Oct4, Sox2, c-Myc and Klf4. hHF-MSC-derived iPSCs appeared indistinguishable from human embryonic stem cells (hESCs) in colony morphology, expression of alkaline phosphotase, and expression of specific hESCs surface markers, SSEA-3, SSEA-4, Tra-1-60, Tra-1-81, Nanog, Oct4, E-Cadherin and endogenous pluripotent genes. When injected into immunocompromised mice, hHF-MSC-derived iPSCs formed teratomas containing representatives of all three germ layers. This is the first study to report reprogramming of hHF-MSCs into iPSCs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12015-012-9420-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-3742959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-37429592013-08-14 Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells Wang, Yimei Liu, Jinyu Tan, Xiaohua Li, Gaofeng Gao, Yunhe Liu, Xuejuan Zhang, Lihong Li, Yulin Stem Cell Rev Article Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) provides an alternative to using embryonic stem cells (ESCs). Mesenchymal stem cells derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking of human hairs. Whether these hHF-MSCs can be reprogrammed has not been previously reported. Here we report the generation of iPSCs from hHF-MSCs obtained by plucking several hairs. hHF-MSCs were isolated from hair follicle tissues and their mesenchymal nature confirmed by detecting cell surface antigens and multilineage differentiation potential towards adipocytes and osteoblasts. They were then reprogrammed into iPSCs by lentiviral transduction with Oct4, Sox2, c-Myc and Klf4. hHF-MSC-derived iPSCs appeared indistinguishable from human embryonic stem cells (hESCs) in colony morphology, expression of alkaline phosphotase, and expression of specific hESCs surface markers, SSEA-3, SSEA-4, Tra-1-60, Tra-1-81, Nanog, Oct4, E-Cadherin and endogenous pluripotent genes. When injected into immunocompromised mice, hHF-MSC-derived iPSCs formed teratomas containing representatives of all three germ layers. This is the first study to report reprogramming of hHF-MSCs into iPSCs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12015-012-9420-5) contains supplementary material, which is available to authorized users. Springer US 2012-12-16 2013 /pmc/articles/PMC3742959/ /pubmed/23242965 http://dx.doi.org/10.1007/s12015-012-9420-5 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Article Wang, Yimei Liu, Jinyu Tan, Xiaohua Li, Gaofeng Gao, Yunhe Liu, Xuejuan Zhang, Lihong Li, Yulin Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells |
title | Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells |
title_full | Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells |
title_fullStr | Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells |
title_full_unstemmed | Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells |
title_short | Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells |
title_sort | induced pluripotent stem cells from human hair follicle mesenchymal stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742959/ https://www.ncbi.nlm.nih.gov/pubmed/23242965 http://dx.doi.org/10.1007/s12015-012-9420-5 |
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