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Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells

Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) provides an alternative to using embryonic stem cells (ESCs). Mesenchymal stem cells derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking of human hairs. Whether these hHF-MSCs...

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Autores principales: Wang, Yimei, Liu, Jinyu, Tan, Xiaohua, Li, Gaofeng, Gao, Yunhe, Liu, Xuejuan, Zhang, Lihong, Li, Yulin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742959/
https://www.ncbi.nlm.nih.gov/pubmed/23242965
http://dx.doi.org/10.1007/s12015-012-9420-5
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author Wang, Yimei
Liu, Jinyu
Tan, Xiaohua
Li, Gaofeng
Gao, Yunhe
Liu, Xuejuan
Zhang, Lihong
Li, Yulin
author_facet Wang, Yimei
Liu, Jinyu
Tan, Xiaohua
Li, Gaofeng
Gao, Yunhe
Liu, Xuejuan
Zhang, Lihong
Li, Yulin
author_sort Wang, Yimei
collection PubMed
description Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) provides an alternative to using embryonic stem cells (ESCs). Mesenchymal stem cells derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking of human hairs. Whether these hHF-MSCs can be reprogrammed has not been previously reported. Here we report the generation of iPSCs from hHF-MSCs obtained by plucking several hairs. hHF-MSCs were isolated from hair follicle tissues and their mesenchymal nature confirmed by detecting cell surface antigens and multilineage differentiation potential towards adipocytes and osteoblasts. They were then reprogrammed into iPSCs by lentiviral transduction with Oct4, Sox2, c-Myc and Klf4. hHF-MSC-derived iPSCs appeared indistinguishable from human embryonic stem cells (hESCs) in colony morphology, expression of alkaline phosphotase, and expression of specific hESCs surface markers, SSEA-3, SSEA-4, Tra-1-60, Tra-1-81, Nanog, Oct4, E-Cadherin and endogenous pluripotent genes. When injected into immunocompromised mice, hHF-MSC-derived iPSCs formed teratomas containing representatives of all three germ layers. This is the first study to report reprogramming of hHF-MSCs into iPSCs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12015-012-9420-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-37429592013-08-14 Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells Wang, Yimei Liu, Jinyu Tan, Xiaohua Li, Gaofeng Gao, Yunhe Liu, Xuejuan Zhang, Lihong Li, Yulin Stem Cell Rev Article Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) provides an alternative to using embryonic stem cells (ESCs). Mesenchymal stem cells derived from human hair follicles (hHF-MSCs) are easily accessible, reproducible by direct plucking of human hairs. Whether these hHF-MSCs can be reprogrammed has not been previously reported. Here we report the generation of iPSCs from hHF-MSCs obtained by plucking several hairs. hHF-MSCs were isolated from hair follicle tissues and their mesenchymal nature confirmed by detecting cell surface antigens and multilineage differentiation potential towards adipocytes and osteoblasts. They were then reprogrammed into iPSCs by lentiviral transduction with Oct4, Sox2, c-Myc and Klf4. hHF-MSC-derived iPSCs appeared indistinguishable from human embryonic stem cells (hESCs) in colony morphology, expression of alkaline phosphotase, and expression of specific hESCs surface markers, SSEA-3, SSEA-4, Tra-1-60, Tra-1-81, Nanog, Oct4, E-Cadherin and endogenous pluripotent genes. When injected into immunocompromised mice, hHF-MSC-derived iPSCs formed teratomas containing representatives of all three germ layers. This is the first study to report reprogramming of hHF-MSCs into iPSCs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12015-012-9420-5) contains supplementary material, which is available to authorized users. Springer US 2012-12-16 2013 /pmc/articles/PMC3742959/ /pubmed/23242965 http://dx.doi.org/10.1007/s12015-012-9420-5 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Wang, Yimei
Liu, Jinyu
Tan, Xiaohua
Li, Gaofeng
Gao, Yunhe
Liu, Xuejuan
Zhang, Lihong
Li, Yulin
Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells
title Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells
title_full Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells
title_fullStr Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells
title_full_unstemmed Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells
title_short Induced Pluripotent Stem Cells from Human Hair Follicle Mesenchymal Stem Cells
title_sort induced pluripotent stem cells from human hair follicle mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742959/
https://www.ncbi.nlm.nih.gov/pubmed/23242965
http://dx.doi.org/10.1007/s12015-012-9420-5
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