A Yeast-Based Chemical Screen Identifies a PDE Inhibitor That Elevates Steroidogenesis in Mouse Leydig Cells via PDE8 and PDE4 Inhibition

A cell-based high-throughput screen (HTS) was developed to detect phosphodiesterase 8 (PDE8) and PDE4/8 combination inhibitors. By replacing the Schizosaccharomyces pombe PDE gene with the murine PDE8A1 gene in strains lacking adenylyl cyclase, we generated strains whose protein kinase A (PKA)-stimu...

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Detalles Bibliográficos
Autores principales: Demirbas, Didem, Wyman, Arlene R., Shimizu-Albergine, Masami, Cakici, Ozgur, Beavo, Joseph A., Hoffman, Charles S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3743849/
https://www.ncbi.nlm.nih.gov/pubmed/23967182
http://dx.doi.org/10.1371/journal.pone.0071279

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3743849/
https://www.ncbi.nlm.nih.gov/pubmed/23967182
http://dx.doi.org/10.1371/journal.pone.0071279

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