Cargando…
Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
Cell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of three-dim...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744071/ https://www.ncbi.nlm.nih.gov/pubmed/23951405 http://dx.doi.org/10.1242/bio.20134564 |
_version_ | 1782280552030666752 |
---|---|
author | Moreira, Carolina G. A. Jacinto, Antonio Prag, Soren |
author_facet | Moreira, Carolina G. A. Jacinto, Antonio Prag, Soren |
author_sort | Moreira, Carolina G. A. |
collection | PubMed |
description | Cell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of three-dimensional cell culture and in vivo systems has shown some differences with what was previously believed to be well-established cell migration mechanisms, suggesting that two-dimensional cell motility would be a poor predictor of in vivo behaviour. Drosophila is a widely recognized model organism to study developmental and homeostatic processes and has been widely used to investigate cell migration. Here, we focus on the migration of small groups of pupal hemocytes that accumulate during larval stages in dorsal patches. We show that integrins, and other known nascent adhesion-related proteins such as Rhea and Fermitin 1, are crucial for this process and that their depletion does not affect polarization in response to environmental cues. We also present evidence for the importance of adhesion maturation-related proteins in hemocyte migration, namely Zyxin. Zyxin depletion in hemocytes leads to a significant increase of cell speed without affecting their response to a chemotactic cue. This is the first report of a systematic analysis using Drosophila melanogaster hemocytes to study adhesion-related proteins and their function in cell migration in vivo. Our data point to mechanisms of cell migration similar to those described in three-dimensional in vitro systems and other in vivo model organisms. |
format | Online Article Text |
id | pubmed-3744071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Company of Biologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-37440712013-08-15 Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo Moreira, Carolina G. A. Jacinto, Antonio Prag, Soren Biol Open Research Article Cell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of three-dimensional cell culture and in vivo systems has shown some differences with what was previously believed to be well-established cell migration mechanisms, suggesting that two-dimensional cell motility would be a poor predictor of in vivo behaviour. Drosophila is a widely recognized model organism to study developmental and homeostatic processes and has been widely used to investigate cell migration. Here, we focus on the migration of small groups of pupal hemocytes that accumulate during larval stages in dorsal patches. We show that integrins, and other known nascent adhesion-related proteins such as Rhea and Fermitin 1, are crucial for this process and that their depletion does not affect polarization in response to environmental cues. We also present evidence for the importance of adhesion maturation-related proteins in hemocyte migration, namely Zyxin. Zyxin depletion in hemocytes leads to a significant increase of cell speed without affecting their response to a chemotactic cue. This is the first report of a systematic analysis using Drosophila melanogaster hemocytes to study adhesion-related proteins and their function in cell migration in vivo. Our data point to mechanisms of cell migration similar to those described in three-dimensional in vitro systems and other in vivo model organisms. The Company of Biologists 2013-06-06 /pmc/articles/PMC3744071/ /pubmed/23951405 http://dx.doi.org/10.1242/bio.20134564 Text en © 2013. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Moreira, Carolina G. A. Jacinto, Antonio Prag, Soren Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
title | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
title_full | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
title_fullStr | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
title_full_unstemmed | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
title_short | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
title_sort | drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744071/ https://www.ncbi.nlm.nih.gov/pubmed/23951405 http://dx.doi.org/10.1242/bio.20134564 |
work_keys_str_mv | AT moreiracarolinaga drosophilaintegrinadhesioncomplexesareessentialforhemocytemigrationinvivo AT jacintoantonio drosophilaintegrinadhesioncomplexesareessentialforhemocytemigrationinvivo AT pragsoren drosophilaintegrinadhesioncomplexesareessentialforhemocytemigrationinvivo |