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Determination of Neuraminidase Kinetic Constants Using Whole Influenza Virus Preparations and Correction for Spectroscopic Interference by a Fluorogenic Substrate
The influenza neuraminidase (NA) enzyme cleaves terminal sialic acid residues from cellular receptors, a process required for the release of newly synthesized virions. A balance of NA activity with sialic acid binding affinity of hemagglutinin (HA) is important for optimal virus replication. NA sequ...
Autores principales: | Marathe, Bindumadhav M., Lévêque, Vincent, Klumpp, Klaus, Webster, Robert G., Govorkova, Elena A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744557/ https://www.ncbi.nlm.nih.gov/pubmed/23977037 http://dx.doi.org/10.1371/journal.pone.0071401 |
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