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Evidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients

Recent application of gene expression profiling to the immune system has shown a great potential for characterization of complex regulatory processes. It is becoming increasingly important to characterize functional systems through multigene interactions to provide valuable insights into differences...

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Detalles Bibliográficos
Autores principales: Dozmorov, Igor, Dominguez, Nicolas, Sestak, Andrea L., Robertson, Julie M., Harley, John B., James, Judith A., Guthridge, Joel M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744560/
https://www.ncbi.nlm.nih.gov/pubmed/23977035
http://dx.doi.org/10.1371/journal.pone.0071397
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author Dozmorov, Igor
Dominguez, Nicolas
Sestak, Andrea L.
Robertson, Julie M.
Harley, John B.
James, Judith A.
Guthridge, Joel M.
author_facet Dozmorov, Igor
Dominguez, Nicolas
Sestak, Andrea L.
Robertson, Julie M.
Harley, John B.
James, Judith A.
Guthridge, Joel M.
author_sort Dozmorov, Igor
collection PubMed
description Recent application of gene expression profiling to the immune system has shown a great potential for characterization of complex regulatory processes. It is becoming increasingly important to characterize functional systems through multigene interactions to provide valuable insights into differences between healthy controls and autoimmune patients. Here we apply an original systematic approach to the analysis of changes in regulatory gene interconnections between in Epstein-Barr virus transformed hyperresponsive B cells from SLE patients and normal control B cells. Both traditional analysis of differential gene expression and analysis of the dynamics of gene expression variations were performed in combination to establish model networks of functional gene expression. This Pathway Dysregulation Analysis identified known transcription factors and transcriptional regulators activated uniquely in stimulated B cells from SLE patients.
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spelling pubmed-37445602013-08-23 Evidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients Dozmorov, Igor Dominguez, Nicolas Sestak, Andrea L. Robertson, Julie M. Harley, John B. James, Judith A. Guthridge, Joel M. PLoS One Research Article Recent application of gene expression profiling to the immune system has shown a great potential for characterization of complex regulatory processes. It is becoming increasingly important to characterize functional systems through multigene interactions to provide valuable insights into differences between healthy controls and autoimmune patients. Here we apply an original systematic approach to the analysis of changes in regulatory gene interconnections between in Epstein-Barr virus transformed hyperresponsive B cells from SLE patients and normal control B cells. Both traditional analysis of differential gene expression and analysis of the dynamics of gene expression variations were performed in combination to establish model networks of functional gene expression. This Pathway Dysregulation Analysis identified known transcription factors and transcriptional regulators activated uniquely in stimulated B cells from SLE patients. Public Library of Science 2013-08-15 /pmc/articles/PMC3744560/ /pubmed/23977035 http://dx.doi.org/10.1371/journal.pone.0071397 Text en © 2013 Dozmorov et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dozmorov, Igor
Dominguez, Nicolas
Sestak, Andrea L.
Robertson, Julie M.
Harley, John B.
James, Judith A.
Guthridge, Joel M.
Evidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients
title Evidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients
title_full Evidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients
title_fullStr Evidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients
title_full_unstemmed Evidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients
title_short Evidence of Dynamically Dysregulated Gene Expression Pathways in Hyperresponsive B Cells from African American Lupus Patients
title_sort evidence of dynamically dysregulated gene expression pathways in hyperresponsive b cells from african american lupus patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744560/
https://www.ncbi.nlm.nih.gov/pubmed/23977035
http://dx.doi.org/10.1371/journal.pone.0071397
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