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Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus

BACKGROUND: Vibrio parahaemolyticus AphA and OpaR are the two master quorum sensing (QS) regulators that are abundantly expressed at low cell density (LCD) and high cell density (HCD), respectively, with a feature of reciprocally gradient production of them with transition between LCD and HCD. The t...

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Autores principales: Wang, Li, Zhou, Dongsheng, Mao, Panyong, Zhang, Yiquan, Hou, Jun, Hu, Yan, Li, Jin, Hou, Shaojie, Yang, Ruifu, Wang, Runhua, Qiu, Jingfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744643/
https://www.ncbi.nlm.nih.gov/pubmed/23977385
http://dx.doi.org/10.1371/journal.pone.0073363
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author Wang, Li
Zhou, Dongsheng
Mao, Panyong
Zhang, Yiquan
Hou, Jun
Hu, Yan
Li, Jin
Hou, Shaojie
Yang, Ruifu
Wang, Runhua
Qiu, Jingfu
author_facet Wang, Li
Zhou, Dongsheng
Mao, Panyong
Zhang, Yiquan
Hou, Jun
Hu, Yan
Li, Jin
Hou, Shaojie
Yang, Ruifu
Wang, Runhua
Qiu, Jingfu
author_sort Wang, Li
collection PubMed
description BACKGROUND: Vibrio parahaemolyticus AphA and OpaR are the two master quorum sensing (QS) regulators that are abundantly expressed at low cell density (LCD) and high cell density (HCD), respectively, with a feature of reciprocally gradient production of them with transition between LCD and HCD. The type VI secretion system 2 (T6SS2) gene cluster can be assigned into three putative operons, namely VPA1027-1024, VPA1043-1028, and VPA1044-1046. T6SS2 contributes to adhesion of V. parahaemolyticus to host cells. METHODOLOGY/PRINCIPAL FINDINGS: OpaR box-like sequences were found within the upstream promoter regions of all the above three operons, while none of AphA box-like elements could be identified for them. The subsequent primer extension, LacZ fusion, electrophoretic mobility shift, and DNase I footprinting assays disclosed that OpaR bound to the promoter regions of these three operons to stimulate their transcription, while AphA negatively regulated their transcription most likely through acting on OpaR. This regulation led to a gradient increase of T6SS2 transcription with transition from LCD to HCD. CONCLUSIONS/SIGNIFICANCE: V. parahaemolyticus OpaR and AphA positively and negatively regulate T6SS2 expression, respectively, leading to a gradient elevation of T6SS2 expression with transition from LCD to HCD. T6SS2 genes are thus assigned as the QS regulon members in V. parahaemolyticus.
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spelling pubmed-37446432013-08-23 Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus Wang, Li Zhou, Dongsheng Mao, Panyong Zhang, Yiquan Hou, Jun Hu, Yan Li, Jin Hou, Shaojie Yang, Ruifu Wang, Runhua Qiu, Jingfu PLoS One Research Article BACKGROUND: Vibrio parahaemolyticus AphA and OpaR are the two master quorum sensing (QS) regulators that are abundantly expressed at low cell density (LCD) and high cell density (HCD), respectively, with a feature of reciprocally gradient production of them with transition between LCD and HCD. The type VI secretion system 2 (T6SS2) gene cluster can be assigned into three putative operons, namely VPA1027-1024, VPA1043-1028, and VPA1044-1046. T6SS2 contributes to adhesion of V. parahaemolyticus to host cells. METHODOLOGY/PRINCIPAL FINDINGS: OpaR box-like sequences were found within the upstream promoter regions of all the above three operons, while none of AphA box-like elements could be identified for them. The subsequent primer extension, LacZ fusion, electrophoretic mobility shift, and DNase I footprinting assays disclosed that OpaR bound to the promoter regions of these three operons to stimulate their transcription, while AphA negatively regulated their transcription most likely through acting on OpaR. This regulation led to a gradient increase of T6SS2 transcription with transition from LCD to HCD. CONCLUSIONS/SIGNIFICANCE: V. parahaemolyticus OpaR and AphA positively and negatively regulate T6SS2 expression, respectively, leading to a gradient elevation of T6SS2 expression with transition from LCD to HCD. T6SS2 genes are thus assigned as the QS regulon members in V. parahaemolyticus. Public Library of Science 2013-08-15 /pmc/articles/PMC3744643/ /pubmed/23977385 http://dx.doi.org/10.1371/journal.pone.0073363 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Li
Zhou, Dongsheng
Mao, Panyong
Zhang, Yiquan
Hou, Jun
Hu, Yan
Li, Jin
Hou, Shaojie
Yang, Ruifu
Wang, Runhua
Qiu, Jingfu
Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus
title Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus
title_full Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus
title_fullStr Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus
title_full_unstemmed Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus
title_short Cell Density- and Quorum Sensing-Dependent Expression of Type VI Secretion System 2 in Vibrio parahaemolyticus
title_sort cell density- and quorum sensing-dependent expression of type vi secretion system 2 in vibrio parahaemolyticus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744643/
https://www.ncbi.nlm.nih.gov/pubmed/23977385
http://dx.doi.org/10.1371/journal.pone.0073363
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