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Dissecting the Catalytic Mechanism of Trypanosoma brucei Trypanothione Synthetase by Kinetic Analysis and Computational Modeling

In pathogenic trypanosomes, trypanothione synthetase (TryS) catalyzes the synthesis of both glutathionylspermidine (Gsp) and trypanothione (bis(glutathionyl)spermidine (T(SH)(2))). Here we present a thorough kinetic analysis of Trypanosoma brucei TryS in a newly developed phosphate buffer system at...

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Detalles Bibliográficos
Autores principales:	Leroux, Alejandro E., Haanstra, Jurgen R., Bakker, Barbara M., Krauth-Siegel, R. Luise
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2013
Materias:	Enzymology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3745322/ https://www.ncbi.nlm.nih.gov/pubmed/23814051 http://dx.doi.org/10.1074/jbc.M113.483289

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