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Effects of Multi-Deficiencies-Diet on Bone Parameters of Peripheral Bone in Ovariectomized Mature Rat
Many postmenopausal women have vitamin D and calcium deficiency. Therefore, vitamin D and calcium supplementation is recommended for all patients with osteopenia and osteoporosis. We used an experimental rat model to test the hypothesis that induction of osteoporosis is more efficiently achieved in...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3745426/ https://www.ncbi.nlm.nih.gov/pubmed/23977109 http://dx.doi.org/10.1371/journal.pone.0071665 |
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author | El Khassawna, Thaqif Böcker, Wolfgang Govindarajan, Parameswari Schliefke, Nathalie Hürter, Britta Kampschulte, Marian Schlewitz, Gudrun Alt, Volker Lips, Katrin Susanne Faulenbach, Miriam Möllmann, Henriette Zahner, Daniel Dürselen, Lutz Ignatius, Anita Bauer, Natali Wenisch, Sabine Langheinrich, Alexander Claus Schnettler, Reinhard Heiss, Christian |
author_facet | El Khassawna, Thaqif Böcker, Wolfgang Govindarajan, Parameswari Schliefke, Nathalie Hürter, Britta Kampschulte, Marian Schlewitz, Gudrun Alt, Volker Lips, Katrin Susanne Faulenbach, Miriam Möllmann, Henriette Zahner, Daniel Dürselen, Lutz Ignatius, Anita Bauer, Natali Wenisch, Sabine Langheinrich, Alexander Claus Schnettler, Reinhard Heiss, Christian |
author_sort | El Khassawna, Thaqif |
collection | PubMed |
description | Many postmenopausal women have vitamin D and calcium deficiency. Therefore, vitamin D and calcium supplementation is recommended for all patients with osteopenia and osteoporosis. We used an experimental rat model to test the hypothesis that induction of osteoporosis is more efficiently achieved in peripheral bone through combining ovariectomy with a unique multi-deficiencies diet (vitamin D depletion and deficient calcium, vitamin K and phosphorus). 14-week-old Sprague-Dawley rats served as controls to examine the initial bone status. 11 rats were bilaterally ovariectomized (OVX) and fed with multi-deficiencies diet. Three months later the treated group and the Sham group (n = 8) were euthanized. Bone biomechanical competence of the diaphyseal bone was examined on both, tibia and femur. Image analysis was performed on tibia via µCT, and on femur via histological analysis. Lower torsional stiffness indicated inferior mechanical competence of the tibia in 3 month OVX+Diet. Proximal metaphyseal region of the tibia showed a diminished bone tissue portion to total tissue in the µCT despite the increased total area as evaluated in both µCT and histology. Cortical bone showed higher porosity and smaller cross sectional thickness of the tibial diaphysis in the OVX+Diet rats. A lower ALP positive area and elevated serum level of RANKL exhibited the unbalanced cellular interaction in bone remodeling in the OVX+Diet rat after 3 month of treatment. Interestingly, more adipose tissue area in bone marrow indicated an effect of bone loss similar to that observed in osteoporotic patients. Nonetheless, the presence of osteoid and elevated serum level of PTH, BGP and Opn suggest the development of osteomalacia rather than an osteoporosis. As the treatment and fracture management of both osteoporotic and osteomalacia patients are clinically overlapping, this study provides a preclinical animal model to be utilized in local supplementation of minerals, drugs and growth factors in future fracture healing studies. |
format | Online Article Text |
id | pubmed-3745426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37454262013-08-23 Effects of Multi-Deficiencies-Diet on Bone Parameters of Peripheral Bone in Ovariectomized Mature Rat El Khassawna, Thaqif Böcker, Wolfgang Govindarajan, Parameswari Schliefke, Nathalie Hürter, Britta Kampschulte, Marian Schlewitz, Gudrun Alt, Volker Lips, Katrin Susanne Faulenbach, Miriam Möllmann, Henriette Zahner, Daniel Dürselen, Lutz Ignatius, Anita Bauer, Natali Wenisch, Sabine Langheinrich, Alexander Claus Schnettler, Reinhard Heiss, Christian PLoS One Research Article Many postmenopausal women have vitamin D and calcium deficiency. Therefore, vitamin D and calcium supplementation is recommended for all patients with osteopenia and osteoporosis. We used an experimental rat model to test the hypothesis that induction of osteoporosis is more efficiently achieved in peripheral bone through combining ovariectomy with a unique multi-deficiencies diet (vitamin D depletion and deficient calcium, vitamin K and phosphorus). 14-week-old Sprague-Dawley rats served as controls to examine the initial bone status. 11 rats were bilaterally ovariectomized (OVX) and fed with multi-deficiencies diet. Three months later the treated group and the Sham group (n = 8) were euthanized. Bone biomechanical competence of the diaphyseal bone was examined on both, tibia and femur. Image analysis was performed on tibia via µCT, and on femur via histological analysis. Lower torsional stiffness indicated inferior mechanical competence of the tibia in 3 month OVX+Diet. Proximal metaphyseal region of the tibia showed a diminished bone tissue portion to total tissue in the µCT despite the increased total area as evaluated in both µCT and histology. Cortical bone showed higher porosity and smaller cross sectional thickness of the tibial diaphysis in the OVX+Diet rats. A lower ALP positive area and elevated serum level of RANKL exhibited the unbalanced cellular interaction in bone remodeling in the OVX+Diet rat after 3 month of treatment. Interestingly, more adipose tissue area in bone marrow indicated an effect of bone loss similar to that observed in osteoporotic patients. Nonetheless, the presence of osteoid and elevated serum level of PTH, BGP and Opn suggest the development of osteomalacia rather than an osteoporosis. As the treatment and fracture management of both osteoporotic and osteomalacia patients are clinically overlapping, this study provides a preclinical animal model to be utilized in local supplementation of minerals, drugs and growth factors in future fracture healing studies. Public Library of Science 2013-08-16 /pmc/articles/PMC3745426/ /pubmed/23977109 http://dx.doi.org/10.1371/journal.pone.0071665 Text en © 2013 El Khassawna et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article El Khassawna, Thaqif Böcker, Wolfgang Govindarajan, Parameswari Schliefke, Nathalie Hürter, Britta Kampschulte, Marian Schlewitz, Gudrun Alt, Volker Lips, Katrin Susanne Faulenbach, Miriam Möllmann, Henriette Zahner, Daniel Dürselen, Lutz Ignatius, Anita Bauer, Natali Wenisch, Sabine Langheinrich, Alexander Claus Schnettler, Reinhard Heiss, Christian Effects of Multi-Deficiencies-Diet on Bone Parameters of Peripheral Bone in Ovariectomized Mature Rat |
title | Effects of Multi-Deficiencies-Diet on Bone Parameters of Peripheral Bone in Ovariectomized Mature Rat |
title_full | Effects of Multi-Deficiencies-Diet on Bone Parameters of Peripheral Bone in Ovariectomized Mature Rat |
title_fullStr | Effects of Multi-Deficiencies-Diet on Bone Parameters of Peripheral Bone in Ovariectomized Mature Rat |
title_full_unstemmed | Effects of Multi-Deficiencies-Diet on Bone Parameters of Peripheral Bone in Ovariectomized Mature Rat |
title_short | Effects of Multi-Deficiencies-Diet on Bone Parameters of Peripheral Bone in Ovariectomized Mature Rat |
title_sort | effects of multi-deficiencies-diet on bone parameters of peripheral bone in ovariectomized mature rat |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3745426/ https://www.ncbi.nlm.nih.gov/pubmed/23977109 http://dx.doi.org/10.1371/journal.pone.0071665 |
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