Cargando…
N-Terminal Domain of Turkey Pancreatic Lipase is Active on Long Chain Triacylglycerols and Stabilized by Colipase
The gene encoding the TPL N-terminal domain (N-TPL), fused with a His6-tag, was cloned and expressed in Pichia pastoris, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAP) constitutive promoter. The recombinant protein was successfully expressed and secreted with an expression l...
Autores principales: | Bou Ali, Madiha, Karray, Aida, Gargouri, Youssef, Ben Ali, Yassine |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3745449/ https://www.ncbi.nlm.nih.gov/pubmed/23977086 http://dx.doi.org/10.1371/journal.pone.0071605 |
Ejemplares similares
-
Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca
por: Ben Bacha, Abir, et al.
Publicado: (2011) -
Purification and characterization of the first recombinant bird pancreatic lipase expressed in Pichia pastoris: The turkey
por: Bou Ali, Madiha, et al.
Publicado: (2011) -
Eukaryotic Expression System Pichia pastoris Affects the Lipase Catalytic Properties: A Monolayer Study
por: Bou Ali, Madiha, et al.
Publicado: (2014) -
N-terminal domain replacement changes an archaeal monoacylglycerol lipase into a triacylglycerol lipase
por: Soni, Surabhi, et al.
Publicado: (2019) -
Dissecting the Interaction Deficiency of a Cartilaginous Fish Digestive Lipase with Pancreatic Colipase: Biochemical and Structural Insights
por: Achouri, Neila, et al.
Publicado: (2020)