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Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast

We previously reported that the increased level of perlecan with altered glycosaminoglycan (GAG) substitution was present in the placenta with gestational diabetes mellitus (GDM) and in the trophoblasts cultured under hyperglycemic condition. Trophoblast is the first cell lineage to differentiate, i...

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Autores principales: Chang, Shu-Chun, Vivian Yang, Wei-Chung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3745874/
https://www.ncbi.nlm.nih.gov/pubmed/23983782
http://dx.doi.org/10.1155/2013/457971
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author Chang, Shu-Chun
Vivian Yang, Wei-Chung
author_facet Chang, Shu-Chun
Vivian Yang, Wei-Chung
author_sort Chang, Shu-Chun
collection PubMed
description We previously reported that the increased level of perlecan with altered glycosaminoglycan (GAG) substitution was present in the placenta with gestational diabetes mellitus (GDM) and in the trophoblasts cultured under hyperglycemic condition. Trophoblast is the first cell lineage to differentiate, invasive, and migrate into the vessel tissues of placenta and fetal membrane during pregnancy. Therefore, active matrix remodeling and vessel formation must occur during placentation. In this study, we further investigated whether hyperglycemia-induced alterations of perlecan in the extracellular matrix (ECM) affect the proliferation and the expressions of angiogenesis-related growth factors and cytokines in the trophoblasts. 3A-Sub-E trophoblastic cells cultured in high glucose medium were conducted to mimic the hyperglycemic condition. Results showed that the hyperglycemia-induced GAG alterations in the cell surface perlecan as well as in the ECM indeed upregulated the expressions of IL-6, IL-8, and MCP-1 and the activities of MMP-2 and MMP-9 and downregulated the expressions of TIMP-2. A regulatory molecular mechanism of hyperglycemia-induced alterations of the cell surface proteoglycans and the ECM remodeling on the expressions of angiogenesis-related cytokines and growth factors in trophoblasts was proposed. This mechanism may contribute to the aberrant placental structure and the maternal and fetal complications during development.
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spelling pubmed-37458742013-08-27 Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast Chang, Shu-Chun Vivian Yang, Wei-Chung Evid Based Complement Alternat Med Research Article We previously reported that the increased level of perlecan with altered glycosaminoglycan (GAG) substitution was present in the placenta with gestational diabetes mellitus (GDM) and in the trophoblasts cultured under hyperglycemic condition. Trophoblast is the first cell lineage to differentiate, invasive, and migrate into the vessel tissues of placenta and fetal membrane during pregnancy. Therefore, active matrix remodeling and vessel formation must occur during placentation. In this study, we further investigated whether hyperglycemia-induced alterations of perlecan in the extracellular matrix (ECM) affect the proliferation and the expressions of angiogenesis-related growth factors and cytokines in the trophoblasts. 3A-Sub-E trophoblastic cells cultured in high glucose medium were conducted to mimic the hyperglycemic condition. Results showed that the hyperglycemia-induced GAG alterations in the cell surface perlecan as well as in the ECM indeed upregulated the expressions of IL-6, IL-8, and MCP-1 and the activities of MMP-2 and MMP-9 and downregulated the expressions of TIMP-2. A regulatory molecular mechanism of hyperglycemia-induced alterations of the cell surface proteoglycans and the ECM remodeling on the expressions of angiogenesis-related cytokines and growth factors in trophoblasts was proposed. This mechanism may contribute to the aberrant placental structure and the maternal and fetal complications during development. Hindawi Publishing Corporation 2013 2013-07-25 /pmc/articles/PMC3745874/ /pubmed/23983782 http://dx.doi.org/10.1155/2013/457971 Text en Copyright © 2013 S.-C. Chang and W.-C. Vivian Yang. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chang, Shu-Chun
Vivian Yang, Wei-Chung
Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
title Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
title_full Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
title_fullStr Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
title_full_unstemmed Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
title_short Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
title_sort hyperglycemia induces altered expressions of angiogenesis associated molecules in the trophoblast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3745874/
https://www.ncbi.nlm.nih.gov/pubmed/23983782
http://dx.doi.org/10.1155/2013/457971
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