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The Function and Significance of SELENBP1 Downregulation in Human Bronchial Epithelial Carcinogenic Process

BACKGROUND: Our quantitative proteomic study showed that selenium-binding protein 1 (SELENBP1) was progressively decreased in human bronchial epithelial carcinogenic process. However, there is little information on expression and function of SELENBP1 during human lung squamous cell cancer (LSCC) car...

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Autores principales: Zeng, Gu-Qing, Yi, Hong, Zhang, Peng-Fei, Li, Xin-Hui, Hu, Rong, Li, Mao-Yu, Li, Cui, Qu, Jia-Quan, Deng, Xingming, Xiao, Zhi-Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747066/
https://www.ncbi.nlm.nih.gov/pubmed/23977169
http://dx.doi.org/10.1371/journal.pone.0071865
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author Zeng, Gu-Qing
Yi, Hong
Zhang, Peng-Fei
Li, Xin-Hui
Hu, Rong
Li, Mao-Yu
Li, Cui
Qu, Jia-Quan
Deng, Xingming
Xiao, Zhi-Qiang
author_facet Zeng, Gu-Qing
Yi, Hong
Zhang, Peng-Fei
Li, Xin-Hui
Hu, Rong
Li, Mao-Yu
Li, Cui
Qu, Jia-Quan
Deng, Xingming
Xiao, Zhi-Qiang
author_sort Zeng, Gu-Qing
collection PubMed
description BACKGROUND: Our quantitative proteomic study showed that selenium-binding protein 1 (SELENBP1) was progressively decreased in human bronchial epithelial carcinogenic process. However, there is little information on expression and function of SELENBP1 during human lung squamous cell cancer (LSCC) carcinogenesis. METHODS: iTRAQ-tagging combined with 2D LC-MS/MS analysis was used to identify differentially expressed proteins in the human bronchial epithelial carcinogenic process. SELENBP1, member of selenoproteins family and progressively downregulated in this process, was selected to further study. Both Western blotting and immunohistochemistry were performed to detect SELENBP1 expression in independent sets of tissues of bronchial epithelial carcinogenesis, and ability of SELENBP1 for discriminating NBE (normal bronchial epithelium) from preneoplastic lesions from invasive LSCC was evaluated. The effects of SELENBP1 downregulation on the susceptibility of benzo(a)pyrene (B[a]P)-induced human bronchial epithelial cell transformation were determined. RESULTS: 102 differentially expressed proteins were identified by quantitative proteomics, and SELENBP1 was found and confirmed being progressively decreased in the human bronchial epithelial carcinogenic process. The sensitivity and specificity of SELENBP1 were 80% and 79% in discriminating NBE from preneoplastic lesions, 79% and 82% in discriminating NBE from invasive LSCC, and 77% and 71% in discriminating preneoplastic lesions from invasive LSCC, respectively. Furthermore, knockdown of SELENBP1 in immortalized human bronchial epithelial cell line 16HBE cells significantly increased the efficiency of B[a]P-induced cell transformation. CONCLUSIONS: The present data shows for the first time that decreased SELENBP1 is an early event in LSCC, increases B[a]P-induced human bronchial epithelial cell transformation, and might serve as a novel potential biomarker for early detection of LSCC.
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spelling pubmed-37470662013-08-23 The Function and Significance of SELENBP1 Downregulation in Human Bronchial Epithelial Carcinogenic Process Zeng, Gu-Qing Yi, Hong Zhang, Peng-Fei Li, Xin-Hui Hu, Rong Li, Mao-Yu Li, Cui Qu, Jia-Quan Deng, Xingming Xiao, Zhi-Qiang PLoS One Research Article BACKGROUND: Our quantitative proteomic study showed that selenium-binding protein 1 (SELENBP1) was progressively decreased in human bronchial epithelial carcinogenic process. However, there is little information on expression and function of SELENBP1 during human lung squamous cell cancer (LSCC) carcinogenesis. METHODS: iTRAQ-tagging combined with 2D LC-MS/MS analysis was used to identify differentially expressed proteins in the human bronchial epithelial carcinogenic process. SELENBP1, member of selenoproteins family and progressively downregulated in this process, was selected to further study. Both Western blotting and immunohistochemistry were performed to detect SELENBP1 expression in independent sets of tissues of bronchial epithelial carcinogenesis, and ability of SELENBP1 for discriminating NBE (normal bronchial epithelium) from preneoplastic lesions from invasive LSCC was evaluated. The effects of SELENBP1 downregulation on the susceptibility of benzo(a)pyrene (B[a]P)-induced human bronchial epithelial cell transformation were determined. RESULTS: 102 differentially expressed proteins were identified by quantitative proteomics, and SELENBP1 was found and confirmed being progressively decreased in the human bronchial epithelial carcinogenic process. The sensitivity and specificity of SELENBP1 were 80% and 79% in discriminating NBE from preneoplastic lesions, 79% and 82% in discriminating NBE from invasive LSCC, and 77% and 71% in discriminating preneoplastic lesions from invasive LSCC, respectively. Furthermore, knockdown of SELENBP1 in immortalized human bronchial epithelial cell line 16HBE cells significantly increased the efficiency of B[a]P-induced cell transformation. CONCLUSIONS: The present data shows for the first time that decreased SELENBP1 is an early event in LSCC, increases B[a]P-induced human bronchial epithelial cell transformation, and might serve as a novel potential biomarker for early detection of LSCC. Public Library of Science 2013-08-19 /pmc/articles/PMC3747066/ /pubmed/23977169 http://dx.doi.org/10.1371/journal.pone.0071865 Text en © 2013 Zeng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zeng, Gu-Qing
Yi, Hong
Zhang, Peng-Fei
Li, Xin-Hui
Hu, Rong
Li, Mao-Yu
Li, Cui
Qu, Jia-Quan
Deng, Xingming
Xiao, Zhi-Qiang
The Function and Significance of SELENBP1 Downregulation in Human Bronchial Epithelial Carcinogenic Process
title The Function and Significance of SELENBP1 Downregulation in Human Bronchial Epithelial Carcinogenic Process
title_full The Function and Significance of SELENBP1 Downregulation in Human Bronchial Epithelial Carcinogenic Process
title_fullStr The Function and Significance of SELENBP1 Downregulation in Human Bronchial Epithelial Carcinogenic Process
title_full_unstemmed The Function and Significance of SELENBP1 Downregulation in Human Bronchial Epithelial Carcinogenic Process
title_short The Function and Significance of SELENBP1 Downregulation in Human Bronchial Epithelial Carcinogenic Process
title_sort function and significance of selenbp1 downregulation in human bronchial epithelial carcinogenic process
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747066/
https://www.ncbi.nlm.nih.gov/pubmed/23977169
http://dx.doi.org/10.1371/journal.pone.0071865
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