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HPat a Decapping Activator Interacting with the miRNA Effector Complex
Animal miRNAs commonly mediate mRNA degradation and/or translational repression by binding to their target mRNAs. Key factors for miRNA-mediated mRNA degradation are the components of the miRNA effector complex (AGO1 and GW182) and the general mRNA degradation machinery (deadenylation and decapping...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747071/ https://www.ncbi.nlm.nih.gov/pubmed/23977167 http://dx.doi.org/10.1371/journal.pone.0071860 |
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author | Barišić-Jäger, Elisabeth Kręcioch, Izabela Hosiner, Stefanie Antic, Sanja Dorner, Silke |
author_facet | Barišić-Jäger, Elisabeth Kręcioch, Izabela Hosiner, Stefanie Antic, Sanja Dorner, Silke |
author_sort | Barišić-Jäger, Elisabeth |
collection | PubMed |
description | Animal miRNAs commonly mediate mRNA degradation and/or translational repression by binding to their target mRNAs. Key factors for miRNA-mediated mRNA degradation are the components of the miRNA effector complex (AGO1 and GW182) and the general mRNA degradation machinery (deadenylation and decapping enzymes). The CCR4-NOT1 complex required for the deadenylation of target mRNAs is directly recruited to the miRNA effector complex. However, it is unclear whether the following decapping step is only a consequence of deadenylation occurring independent of the miRNA effector complex or e.g. decapping activators can get recruited to the miRNA effector complex. In this study we performed split-affinity purifications in Drosophila cells and provide evidence for the interaction of the decapping activator HPat with the miRNA effector complex. Furthermore, in knockdown analysis of various mRNA degradation factors we demonstrate the importance of NOT1 for this interaction. This suggests that deadenylation and/or the recruitment of NOT1 protein precedes the association of HPat with the miRNA effector complex. Since HPat couples deadenylation and decapping, the recruitment of HPat to the miRNA effector complex provides a mechanism to commit the mRNA target for degradation. |
format | Online Article Text |
id | pubmed-3747071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37470712013-08-23 HPat a Decapping Activator Interacting with the miRNA Effector Complex Barišić-Jäger, Elisabeth Kręcioch, Izabela Hosiner, Stefanie Antic, Sanja Dorner, Silke PLoS One Research Article Animal miRNAs commonly mediate mRNA degradation and/or translational repression by binding to their target mRNAs. Key factors for miRNA-mediated mRNA degradation are the components of the miRNA effector complex (AGO1 and GW182) and the general mRNA degradation machinery (deadenylation and decapping enzymes). The CCR4-NOT1 complex required for the deadenylation of target mRNAs is directly recruited to the miRNA effector complex. However, it is unclear whether the following decapping step is only a consequence of deadenylation occurring independent of the miRNA effector complex or e.g. decapping activators can get recruited to the miRNA effector complex. In this study we performed split-affinity purifications in Drosophila cells and provide evidence for the interaction of the decapping activator HPat with the miRNA effector complex. Furthermore, in knockdown analysis of various mRNA degradation factors we demonstrate the importance of NOT1 for this interaction. This suggests that deadenylation and/or the recruitment of NOT1 protein precedes the association of HPat with the miRNA effector complex. Since HPat couples deadenylation and decapping, the recruitment of HPat to the miRNA effector complex provides a mechanism to commit the mRNA target for degradation. Public Library of Science 2013-08-19 /pmc/articles/PMC3747071/ /pubmed/23977167 http://dx.doi.org/10.1371/journal.pone.0071860 Text en © 2013 Barišić-Jäger et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Barišić-Jäger, Elisabeth Kręcioch, Izabela Hosiner, Stefanie Antic, Sanja Dorner, Silke HPat a Decapping Activator Interacting with the miRNA Effector Complex |
title | HPat a Decapping Activator Interacting with the miRNA Effector Complex |
title_full | HPat a Decapping Activator Interacting with the miRNA Effector Complex |
title_fullStr | HPat a Decapping Activator Interacting with the miRNA Effector Complex |
title_full_unstemmed | HPat a Decapping Activator Interacting with the miRNA Effector Complex |
title_short | HPat a Decapping Activator Interacting with the miRNA Effector Complex |
title_sort | hpat a decapping activator interacting with the mirna effector complex |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747071/ https://www.ncbi.nlm.nih.gov/pubmed/23977167 http://dx.doi.org/10.1371/journal.pone.0071860 |
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