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Acute Physiological Stress Down-Regulates mRNA Expressions of Growth-Related Genes in Coho Salmon

Growth and development in fish are regulated to a major extent by growth-related factors, such as liver-derived insulin-like growth factor (IGF) -1 in response to pituitary-secreted growth hormone (GH) binding to the GH receptor (GHR). Here, we report on the changes in the expressions of gh, ghr, an...

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Autores principales: Nakano, Toshiki, Afonso, Luis O. B., Beckman, Brian R., Iwama, George K., Devlin, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747168/
https://www.ncbi.nlm.nih.gov/pubmed/23990952
http://dx.doi.org/10.1371/journal.pone.0071421
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author Nakano, Toshiki
Afonso, Luis O. B.
Beckman, Brian R.
Iwama, George K.
Devlin, Robert H.
author_facet Nakano, Toshiki
Afonso, Luis O. B.
Beckman, Brian R.
Iwama, George K.
Devlin, Robert H.
author_sort Nakano, Toshiki
collection PubMed
description Growth and development in fish are regulated to a major extent by growth-related factors, such as liver-derived insulin-like growth factor (IGF) -1 in response to pituitary-secreted growth hormone (GH) binding to the GH receptor (GHR). Here, we report on the changes in the expressions of gh, ghr, and igf1 genes and the circulating levels of GH and IGF-1 proteins in juvenile coho salmon (Oncorhynchus kisutch) in response to handling as an acute physiological stressor. Plasma GH levels were not significantly different between stressed fish and prestressed control. Plasma IGF-1 concentrations in stressed fish 1.5 h post-stress were the same as in control fish, but levels in stressed fish decreased significantly 16 h post-stress. Real-time quantitative PCR (qPCR) analysis showed that ghr mRNA levels in pituitary, liver, and muscle decreased gradually in response to the stressor. After exposure to stress, hepatic igf1 expression transiently increased, whereas levels decreased 16 h post-stress. On the other hand, the pituitary gh mRNA level did not change in response to the stressor. These observations indicate that expression of gh, ghr, and igf1 responded differently to stress. Our results show that acute physiological stress can mainly down-regulate the expressions of growth-related genes in coho salmon in vivo. This study also suggests that a relationship between the neuroendocrine stress response and growth-related factors exists in fish.
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spelling pubmed-37471682013-08-29 Acute Physiological Stress Down-Regulates mRNA Expressions of Growth-Related Genes in Coho Salmon Nakano, Toshiki Afonso, Luis O. B. Beckman, Brian R. Iwama, George K. Devlin, Robert H. PLoS One Research Article Growth and development in fish are regulated to a major extent by growth-related factors, such as liver-derived insulin-like growth factor (IGF) -1 in response to pituitary-secreted growth hormone (GH) binding to the GH receptor (GHR). Here, we report on the changes in the expressions of gh, ghr, and igf1 genes and the circulating levels of GH and IGF-1 proteins in juvenile coho salmon (Oncorhynchus kisutch) in response to handling as an acute physiological stressor. Plasma GH levels were not significantly different between stressed fish and prestressed control. Plasma IGF-1 concentrations in stressed fish 1.5 h post-stress were the same as in control fish, but levels in stressed fish decreased significantly 16 h post-stress. Real-time quantitative PCR (qPCR) analysis showed that ghr mRNA levels in pituitary, liver, and muscle decreased gradually in response to the stressor. After exposure to stress, hepatic igf1 expression transiently increased, whereas levels decreased 16 h post-stress. On the other hand, the pituitary gh mRNA level did not change in response to the stressor. These observations indicate that expression of gh, ghr, and igf1 responded differently to stress. Our results show that acute physiological stress can mainly down-regulate the expressions of growth-related genes in coho salmon in vivo. This study also suggests that a relationship between the neuroendocrine stress response and growth-related factors exists in fish. Public Library of Science 2013-08-19 /pmc/articles/PMC3747168/ /pubmed/23990952 http://dx.doi.org/10.1371/journal.pone.0071421 Text en © 2013 Nakano et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nakano, Toshiki
Afonso, Luis O. B.
Beckman, Brian R.
Iwama, George K.
Devlin, Robert H.
Acute Physiological Stress Down-Regulates mRNA Expressions of Growth-Related Genes in Coho Salmon
title Acute Physiological Stress Down-Regulates mRNA Expressions of Growth-Related Genes in Coho Salmon
title_full Acute Physiological Stress Down-Regulates mRNA Expressions of Growth-Related Genes in Coho Salmon
title_fullStr Acute Physiological Stress Down-Regulates mRNA Expressions of Growth-Related Genes in Coho Salmon
title_full_unstemmed Acute Physiological Stress Down-Regulates mRNA Expressions of Growth-Related Genes in Coho Salmon
title_short Acute Physiological Stress Down-Regulates mRNA Expressions of Growth-Related Genes in Coho Salmon
title_sort acute physiological stress down-regulates mrna expressions of growth-related genes in coho salmon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747168/
https://www.ncbi.nlm.nih.gov/pubmed/23990952
http://dx.doi.org/10.1371/journal.pone.0071421
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