Impact of Library Preparation on Downstream Analysis and Interpretation of RNA-Seq Data: Comparison between Illumina PolyA and NuGEN Ovation Protocol

OBJECTIVES: The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses...

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Detalles Bibliográficos
Autores principales: Sun, Zhifu, Asmann, Yan W., Nair, Asha, Zhang, Yuji, Wang, Liguo, Kalari, Krishna R., Bhagwate, Aditya V., Baker, Tiffany R., Carr, Jennifer M., Kocher, Jean-Pierre A., Perez, Edith A., Thompson, E. Aubrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747248/
https://www.ncbi.nlm.nih.gov/pubmed/23977132
http://dx.doi.org/10.1371/journal.pone.0071745

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