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Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores

Miscanthus × giganteus is a popular energy crop, which due to its hybrid origin is only vegetatively reproduced. Asexual embryogenesis in anther and microspore culture leading to double haploids production could allow to regain the ability for sexual reproduction and to increase the biodiversity of...

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Autores principales: Żur, Iwona, Dubas, Ewa, Słomka, Aneta, Dubert, Franciszek, Kuta, Elżbieta, Płażek, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747323/
https://www.ncbi.nlm.nih.gov/pubmed/23824237
http://dx.doi.org/10.1007/s00497-013-0219-6
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author Żur, Iwona
Dubas, Ewa
Słomka, Aneta
Dubert, Franciszek
Kuta, Elżbieta
Płażek, Agnieszka
author_facet Żur, Iwona
Dubas, Ewa
Słomka, Aneta
Dubert, Franciszek
Kuta, Elżbieta
Płażek, Agnieszka
author_sort Żur, Iwona
collection PubMed
description Miscanthus × giganteus is a popular energy crop, which due to its hybrid origin is only vegetatively reproduced. Asexual embryogenesis in anther and microspore culture leading to double haploids production could allow to regain the ability for sexual reproduction and to increase the biodiversity of the species. Therefore, the goal of this paper was to investigate the requirements of androgenesis in Miscanthus. The standard protocols used for monocotyledonous plants were applied with many modifications regarding the developmental stage of the explants at the time of culture initiation, stress treatment applied to panicles and isolated anthers as well as various chemical and physical parameters of in vitro culture conditions. Our results indicated that the induction of androgenesis in M. × giganteus is possible. However, the very low efficiency of the process and the lack of regeneration ability of the androgenic structures presently prevent the use of this technique.
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spelling pubmed-37473232013-08-20 Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores Żur, Iwona Dubas, Ewa Słomka, Aneta Dubert, Franciszek Kuta, Elżbieta Płażek, Agnieszka Plant Reprod Original Article Miscanthus × giganteus is a popular energy crop, which due to its hybrid origin is only vegetatively reproduced. Asexual embryogenesis in anther and microspore culture leading to double haploids production could allow to regain the ability for sexual reproduction and to increase the biodiversity of the species. Therefore, the goal of this paper was to investigate the requirements of androgenesis in Miscanthus. The standard protocols used for monocotyledonous plants were applied with many modifications regarding the developmental stage of the explants at the time of culture initiation, stress treatment applied to panicles and isolated anthers as well as various chemical and physical parameters of in vitro culture conditions. Our results indicated that the induction of androgenesis in M. × giganteus is possible. However, the very low efficiency of the process and the lack of regeneration ability of the androgenic structures presently prevent the use of this technique. Springer Berlin Heidelberg 2013-07-04 2013 /pmc/articles/PMC3747323/ /pubmed/23824237 http://dx.doi.org/10.1007/s00497-013-0219-6 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Żur, Iwona
Dubas, Ewa
Słomka, Aneta
Dubert, Franciszek
Kuta, Elżbieta
Płażek, Agnieszka
Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores
title Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores
title_full Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores
title_fullStr Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores
title_full_unstemmed Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores
title_short Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores
title_sort failure of androgenesis in miscanthus × giganteus in vitro culture of cytologically unbalanced microspores
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747323/
https://www.ncbi.nlm.nih.gov/pubmed/23824237
http://dx.doi.org/10.1007/s00497-013-0219-6
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