Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells

Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression of β-integrin, CD44, CD71, and CD73 was obser...

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Detalles Bibliográficos
Autores principales: Gao, Yuhua, Bai, Chunyu, Xiong, Hui, Li, Qian, Shan, Zhiqiang, Huang, Linsheng, Ma, Yuehui, Guan, Weijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747373/
https://www.ncbi.nlm.nih.gov/pubmed/23984389
http://dx.doi.org/10.1155/2013/626258
Descripción
Sumario:Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression of β-integrin, CD44, CD71, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 DMS/PCs were successfully induced to differentiate into osteoblasts, adipocytes, and neurocytes. The results indicate the potential for multilineage differentiation of DMS/PCs that may represent an ideal candidate for cellular transplantation therapy.

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