Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells

Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression of β-integrin, CD44, CD71, and CD73 was obser...

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Autores principales: Gao, Yuhua, Bai, Chunyu, Xiong, Hui, Li, Qian, Shan, Zhiqiang, Huang, Linsheng, Ma, Yuehui, Guan, Weijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747373/
https://www.ncbi.nlm.nih.gov/pubmed/23984389
http://dx.doi.org/10.1155/2013/626258
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author Gao, Yuhua
Bai, Chunyu
Xiong, Hui
Li, Qian
Shan, Zhiqiang
Huang, Linsheng
Ma, Yuehui
Guan, Weijun
author_facet Gao, Yuhua
Bai, Chunyu
Xiong, Hui
Li, Qian
Shan, Zhiqiang
Huang, Linsheng
Ma, Yuehui
Guan, Weijun
author_sort Gao, Yuhua
collection PubMed
description Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression of β-integrin, CD44, CD71, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 DMS/PCs were successfully induced to differentiate into osteoblasts, adipocytes, and neurocytes. The results indicate the potential for multilineage differentiation of DMS/PCs that may represent an ideal candidate for cellular transplantation therapy.
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spelling pubmed-37473732013-08-27 Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells Gao, Yuhua Bai, Chunyu Xiong, Hui Li, Qian Shan, Zhiqiang Huang, Linsheng Ma, Yuehui Guan, Weijun Biomed Res Int Research Article Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression of β-integrin, CD44, CD71, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 DMS/PCs were successfully induced to differentiate into osteoblasts, adipocytes, and neurocytes. The results indicate the potential for multilineage differentiation of DMS/PCs that may represent an ideal candidate for cellular transplantation therapy. Hindawi Publishing Corporation 2013 2013-08-04 /pmc/articles/PMC3747373/ /pubmed/23984389 http://dx.doi.org/10.1155/2013/626258 Text en Copyright © 2013 Yuhua Gao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gao, Yuhua
Bai, Chunyu
Xiong, Hui
Li, Qian
Shan, Zhiqiang
Huang, Linsheng
Ma, Yuehui
Guan, Weijun
Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
title Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
title_full Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
title_fullStr Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
title_full_unstemmed Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
title_short Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
title_sort isolation and characterization of chicken dermis-derived mesenchymal stem/progenitor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747373/
https://www.ncbi.nlm.nih.gov/pubmed/23984389
http://dx.doi.org/10.1155/2013/626258
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