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Reliable HPLC Determination of Aflatoxin M1 in Eggs
Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747390/ https://www.ncbi.nlm.nih.gov/pubmed/23984192 http://dx.doi.org/10.1155/2013/817091 |
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author | Khalil, Mostafa M. H. Gomaa, Ahmed M. Sebaei, Ahmed Salem |
author_facet | Khalil, Mostafa M. H. Gomaa, Ahmed M. Sebaei, Ahmed Salem |
author_sort | Khalil, Mostafa M. H. |
collection | PubMed |
description | Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg samples were diluted with warmed water and the toxin was immunoextracted followed by fluorescence detection. The average recovery of aflatoxin M1 at the three different levels 0.05, 0.1, and 0.5 μg/kg varied between 87% and 98%. The method is linear from the limit of quantification 0.05 μg/kg up to 3 μg/kg levels. This method is intended for aflatoxin M1 analyses in eggs simply with minimum toxin lose, excellent recovery, and accurate results with the limit of detection 0.01 μg/kg. |
format | Online Article Text |
id | pubmed-3747390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37473902013-08-27 Reliable HPLC Determination of Aflatoxin M1 in Eggs Khalil, Mostafa M. H. Gomaa, Ahmed M. Sebaei, Ahmed Salem J Anal Methods Chem Research Article Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in eggs was described. The egg samples were diluted with warmed water and the toxin was immunoextracted followed by fluorescence detection. The average recovery of aflatoxin M1 at the three different levels 0.05, 0.1, and 0.5 μg/kg varied between 87% and 98%. The method is linear from the limit of quantification 0.05 μg/kg up to 3 μg/kg levels. This method is intended for aflatoxin M1 analyses in eggs simply with minimum toxin lose, excellent recovery, and accurate results with the limit of detection 0.01 μg/kg. Hindawi Publishing Corporation 2013 2013-08-01 /pmc/articles/PMC3747390/ /pubmed/23984192 http://dx.doi.org/10.1155/2013/817091 Text en Copyright © 2013 Mostafa M. H. Khalil et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Khalil, Mostafa M. H. Gomaa, Ahmed M. Sebaei, Ahmed Salem Reliable HPLC Determination of Aflatoxin M1 in Eggs |
title | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
title_full | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
title_fullStr | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
title_full_unstemmed | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
title_short | Reliable HPLC Determination of Aflatoxin M1 in Eggs |
title_sort | reliable hplc determination of aflatoxin m1 in eggs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747390/ https://www.ncbi.nlm.nih.gov/pubmed/23984192 http://dx.doi.org/10.1155/2013/817091 |
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