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Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration

Nitric oxide (NO) found in the vicinity of lung cancer cells may play a role in the regulation of cancer cell behaviors. To explore the possible effects of NO on cell motility, human lung cancer cells were exposed to nontoxic concentrations of NO for 0–14 days, and the migratory characteristics of t...

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Autores principales: Sanuphan, Arpasinee, Chunhacha, Preedakorn, Pongrakhananon, Varisa, Chanvorachote, Pithi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747486/
https://www.ncbi.nlm.nih.gov/pubmed/23984323
http://dx.doi.org/10.1155/2013/186972
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author Sanuphan, Arpasinee
Chunhacha, Preedakorn
Pongrakhananon, Varisa
Chanvorachote, Pithi
author_facet Sanuphan, Arpasinee
Chunhacha, Preedakorn
Pongrakhananon, Varisa
Chanvorachote, Pithi
author_sort Sanuphan, Arpasinee
collection PubMed
description Nitric oxide (NO) found in the vicinity of lung cancer cells may play a role in the regulation of cancer cell behaviors. To explore the possible effects of NO on cell motility, human lung cancer cells were exposed to nontoxic concentrations of NO for 0–14 days, and the migratory characteristics of the cells were determined. The present study found that long-term treatment with NO significantly enhanced cell migration in a dose- and time-dependent manner. Furthermore, we found that the increased migratory action was associated with the increased expression of caveolin-1 (Cav-1), which in turn activated the focal adhesion kinase (FAK) and ATP-dependent tyrosine kinase (Akt) pathways. Notably, the NO-treated cells exhibited an increased number of filopodia per cell, as well as an increase in the levels of cell division cycle 42 (Cdc42) protein. Together, these results indicate that extended NO exposure has a novel effect on cell migration through a Cav-1-dependent mechanism, a finding that strengthens our understanding of cancer biology.
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spelling pubmed-37474862013-08-27 Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration Sanuphan, Arpasinee Chunhacha, Preedakorn Pongrakhananon, Varisa Chanvorachote, Pithi Biomed Res Int Research Article Nitric oxide (NO) found in the vicinity of lung cancer cells may play a role in the regulation of cancer cell behaviors. To explore the possible effects of NO on cell motility, human lung cancer cells were exposed to nontoxic concentrations of NO for 0–14 days, and the migratory characteristics of the cells were determined. The present study found that long-term treatment with NO significantly enhanced cell migration in a dose- and time-dependent manner. Furthermore, we found that the increased migratory action was associated with the increased expression of caveolin-1 (Cav-1), which in turn activated the focal adhesion kinase (FAK) and ATP-dependent tyrosine kinase (Akt) pathways. Notably, the NO-treated cells exhibited an increased number of filopodia per cell, as well as an increase in the levels of cell division cycle 42 (Cdc42) protein. Together, these results indicate that extended NO exposure has a novel effect on cell migration through a Cav-1-dependent mechanism, a finding that strengthens our understanding of cancer biology. Hindawi Publishing Corporation 2013 2013-07-31 /pmc/articles/PMC3747486/ /pubmed/23984323 http://dx.doi.org/10.1155/2013/186972 Text en Copyright © 2013 Arpasinee Sanuphan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sanuphan, Arpasinee
Chunhacha, Preedakorn
Pongrakhananon, Varisa
Chanvorachote, Pithi
Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration
title Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration
title_full Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration
title_fullStr Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration
title_full_unstemmed Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration
title_short Long-Term Nitric Oxide Exposure Enhances Lung Cancer Cell Migration
title_sort long-term nitric oxide exposure enhances lung cancer cell migration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747486/
https://www.ncbi.nlm.nih.gov/pubmed/23984323
http://dx.doi.org/10.1155/2013/186972
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