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Expression of Stem Cell Markers in Primo Vessel of Rat

Accumulating line of evidence support that adult tissues contain a rare population of pluripotent stem cells (PSCs), which differentiate into all types of cells in our body. Bonghan microcell (primo microcells (PMCs)) discovered in 1960s was reported to have a pluripotency like a stem cell in vivo a...

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Autores principales: Park, Eun Seok, Lee, Jeong Hoon, Kim, Won Jin, Heo, Jinbeom, Shin, Dong Myung, Leem, Chae Hun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747494/
https://www.ncbi.nlm.nih.gov/pubmed/23983780
http://dx.doi.org/10.1155/2013/438079
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author Park, Eun Seok
Lee, Jeong Hoon
Kim, Won Jin
Heo, Jinbeom
Shin, Dong Myung
Leem, Chae Hun
author_facet Park, Eun Seok
Lee, Jeong Hoon
Kim, Won Jin
Heo, Jinbeom
Shin, Dong Myung
Leem, Chae Hun
author_sort Park, Eun Seok
collection PubMed
description Accumulating line of evidence support that adult tissues contain a rare population of pluripotent stem cells (PSCs), which differentiate into all types of cells in our body. Bonghan microcell (primo microcells (PMCs)) discovered in 1960s was reported to have a pluripotency like a stem cell in vivo as well as in vitro condition. Here, we describe the detailed morphology and molecular features of PMCs. PMCs reside in Bonghan duct (primo vessel (PV)) reported as a corresponding structure of acupuncture points and meridian system. We found that PMCs were frequently observed in the liver surface of the rat between 300 g and 400 g from April to June, suggesting that the their detection frequency depends on the weight, the season, and the organ of rat. As reported, PMCs freshly isolated from PVs were spherical ~1-2 μm microsized cells. In contrast, a unique bithread or budding-shaped PMCs emerged during tissue culture around 8 days. RT-PCR analysis demonstrated that PVs-derived cells express the Oct4, the most important PSCs gene, in addition to several PSCs markers (Sox2, Stella, Rex1, and Klf4). Thus, we for the first time provide the evidence about Oct4-expressing stem-like characteristics for cells resident in PVs, a possible novel stem cell enriched niche.
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spelling pubmed-37474942013-08-27 Expression of Stem Cell Markers in Primo Vessel of Rat Park, Eun Seok Lee, Jeong Hoon Kim, Won Jin Heo, Jinbeom Shin, Dong Myung Leem, Chae Hun Evid Based Complement Alternat Med Research Article Accumulating line of evidence support that adult tissues contain a rare population of pluripotent stem cells (PSCs), which differentiate into all types of cells in our body. Bonghan microcell (primo microcells (PMCs)) discovered in 1960s was reported to have a pluripotency like a stem cell in vivo as well as in vitro condition. Here, we describe the detailed morphology and molecular features of PMCs. PMCs reside in Bonghan duct (primo vessel (PV)) reported as a corresponding structure of acupuncture points and meridian system. We found that PMCs were frequently observed in the liver surface of the rat between 300 g and 400 g from April to June, suggesting that the their detection frequency depends on the weight, the season, and the organ of rat. As reported, PMCs freshly isolated from PVs were spherical ~1-2 μm microsized cells. In contrast, a unique bithread or budding-shaped PMCs emerged during tissue culture around 8 days. RT-PCR analysis demonstrated that PVs-derived cells express the Oct4, the most important PSCs gene, in addition to several PSCs markers (Sox2, Stella, Rex1, and Klf4). Thus, we for the first time provide the evidence about Oct4-expressing stem-like characteristics for cells resident in PVs, a possible novel stem cell enriched niche. Hindawi Publishing Corporation 2013 2013-07-31 /pmc/articles/PMC3747494/ /pubmed/23983780 http://dx.doi.org/10.1155/2013/438079 Text en Copyright © 2013 Eun Seok Park et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Park, Eun Seok
Lee, Jeong Hoon
Kim, Won Jin
Heo, Jinbeom
Shin, Dong Myung
Leem, Chae Hun
Expression of Stem Cell Markers in Primo Vessel of Rat
title Expression of Stem Cell Markers in Primo Vessel of Rat
title_full Expression of Stem Cell Markers in Primo Vessel of Rat
title_fullStr Expression of Stem Cell Markers in Primo Vessel of Rat
title_full_unstemmed Expression of Stem Cell Markers in Primo Vessel of Rat
title_short Expression of Stem Cell Markers in Primo Vessel of Rat
title_sort expression of stem cell markers in primo vessel of rat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747494/
https://www.ncbi.nlm.nih.gov/pubmed/23983780
http://dx.doi.org/10.1155/2013/438079
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