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Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012

OBJECTIVES: In this study, we demonstrated that TBEV-infected ticks have been distributed in the ROK, combined with our previous results. These results suggest that TBEV may exist in the ROK, and H. longicornis, H. flava, and I. nipponensis may be potential vectors of TBEV. In addition, these result...

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Autores principales: Yun, Seok-Min, Song, Bong Gu, Choi, WooYoung, Park, Won Il, Kim, Sung Yun, Roh, Jong Yul, Ryou, Jungsang, Ju, Young Ran, Park, Chan, Shin, E-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korea Centers for Disease Control and Prevention 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747658/
https://www.ncbi.nlm.nih.gov/pubmed/24159517
http://dx.doi.org/10.1016/j.phrp.2012.10.004
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author Yun, Seok-Min
Song, Bong Gu
Choi, WooYoung
Park, Won Il
Kim, Sung Yun
Roh, Jong Yul
Ryou, Jungsang
Ju, Young Ran
Park, Chan
Shin, E-Hyun
author_facet Yun, Seok-Min
Song, Bong Gu
Choi, WooYoung
Park, Won Il
Kim, Sung Yun
Roh, Jong Yul
Ryou, Jungsang
Ju, Young Ran
Park, Chan
Shin, E-Hyun
author_sort Yun, Seok-Min
collection PubMed
description OBJECTIVES: In this study, we demonstrated that TBEV-infected ticks have been distributed in the ROK, combined with our previous results. These results suggest that TBEV may exist in the ROK, and H. longicornis, H. flava, and I. nipponensis may be potential vectors of TBEV. In addition, these results emphasize the need for further epidemiological research of TBEV. METHODS: We examined for the presence of RNA of TBEV by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) using ixodid ticks captured in 25 localities of 10 provinces. Ticks were collected by the flagging and dragging method or using sentinel BG traps at forests, grass thickets, and grassland. A total of 13,053 ticks belonging to two genera and four species were collected and pooled (1292 pools), according to collection site, species of tick, and developmental stage. RESULTS: Among 1292 pools, the envelope (E) protein gene of TBEV was detected using RT-nested PCR in 10 pools (3 pools of the 1,331 adult ticks and 7 pools of the 11,169 nymph ticks) collected from Gangwon-do province, Jeonrabuk-do province, and Jeju Island. The minimum infection rates for TBEV of Haemaphysalis longicornis, Haemaphysalis flava, and Ixodes nipponensis were 0.06%, 0.17%, and 2.38%, respectively. Phylogenetic analysis based on the partial E protein gene was performed to identify relationships between the TBEV strains. This showed that 10 Korean strains clustered with the Western subtype. CONCLUSION: In this study, we investigated the prevalence of tick-borne encephalitis virus (TBEV) in ixodid ticks from various regions of the Republic of Korea (ROK) during 2011–2012 to identify whether TBEV is circulating and to determine the endemic regions of TBEV.
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spelling pubmed-37476582013-10-24 Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012 Yun, Seok-Min Song, Bong Gu Choi, WooYoung Park, Won Il Kim, Sung Yun Roh, Jong Yul Ryou, Jungsang Ju, Young Ran Park, Chan Shin, E-Hyun Osong Public Health Res Perspect Articles OBJECTIVES: In this study, we demonstrated that TBEV-infected ticks have been distributed in the ROK, combined with our previous results. These results suggest that TBEV may exist in the ROK, and H. longicornis, H. flava, and I. nipponensis may be potential vectors of TBEV. In addition, these results emphasize the need for further epidemiological research of TBEV. METHODS: We examined for the presence of RNA of TBEV by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) using ixodid ticks captured in 25 localities of 10 provinces. Ticks were collected by the flagging and dragging method or using sentinel BG traps at forests, grass thickets, and grassland. A total of 13,053 ticks belonging to two genera and four species were collected and pooled (1292 pools), according to collection site, species of tick, and developmental stage. RESULTS: Among 1292 pools, the envelope (E) protein gene of TBEV was detected using RT-nested PCR in 10 pools (3 pools of the 1,331 adult ticks and 7 pools of the 11,169 nymph ticks) collected from Gangwon-do province, Jeonrabuk-do province, and Jeju Island. The minimum infection rates for TBEV of Haemaphysalis longicornis, Haemaphysalis flava, and Ixodes nipponensis were 0.06%, 0.17%, and 2.38%, respectively. Phylogenetic analysis based on the partial E protein gene was performed to identify relationships between the TBEV strains. This showed that 10 Korean strains clustered with the Western subtype. CONCLUSION: In this study, we investigated the prevalence of tick-borne encephalitis virus (TBEV) in ixodid ticks from various regions of the Republic of Korea (ROK) during 2011–2012 to identify whether TBEV is circulating and to determine the endemic regions of TBEV. Korea Centers for Disease Control and Prevention 2012-12 /pmc/articles/PMC3747658/ /pubmed/24159517 http://dx.doi.org/10.1016/j.phrp.2012.10.004 Text en Copyright ©2012, Korea Centers for Disease Control and Prevention http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:// creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Yun, Seok-Min
Song, Bong Gu
Choi, WooYoung
Park, Won Il
Kim, Sung Yun
Roh, Jong Yul
Ryou, Jungsang
Ju, Young Ran
Park, Chan
Shin, E-Hyun
Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012
title Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012
title_full Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012
title_fullStr Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012
title_full_unstemmed Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012
title_short Prevalence of Tick-Borne Encephalitis Virus in Ixodid Ticks Collected from the Republic of Korea During 2011–2012
title_sort prevalence of tick-borne encephalitis virus in ixodid ticks collected from the republic of korea during 2011–2012
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747658/
https://www.ncbi.nlm.nih.gov/pubmed/24159517
http://dx.doi.org/10.1016/j.phrp.2012.10.004
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