Cargando…
Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8)
OBJECTIVES: We aimed at evaluating the virulence of atypical Shigella flexneri II:(3)4,7(8) by DNA microarray and invasion assay. METHODS: We used a customized S. flexneri DNA microarray to analyze an atypical S. flexneri II:(3)4,7(8) gene expression profile and compared it with that of the S. flexn...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korea Centers for Disease Control and Prevention
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747661/ https://www.ncbi.nlm.nih.gov/pubmed/24159518 http://dx.doi.org/10.1016/j.phrp.2012.10.002 |
_version_ | 1782280969813753856 |
---|---|
author | Hong, Sahyun Cha, Injun Kim, Nan-Ok Kim, Seong-Han Jung, Kyung-Tae Lee, Je-Hee Kim, Dong-Wook Park, Mi-Sun Kang, Yeon-Ho |
author_facet | Hong, Sahyun Cha, Injun Kim, Nan-Ok Kim, Seong-Han Jung, Kyung-Tae Lee, Je-Hee Kim, Dong-Wook Park, Mi-Sun Kang, Yeon-Ho |
author_sort | Hong, Sahyun |
collection | PubMed |
description | OBJECTIVES: We aimed at evaluating the virulence of atypical Shigella flexneri II:(3)4,7(8) by DNA microarray and invasion assay. METHODS: We used a customized S. flexneri DNA microarray to analyze an atypical S. flexneri II:(3)4,7(8) gene expression profile and compared it with that of the S. flexneri 2b strain. RESULTS: Approximately one-quarter of the atypical S. flexneri II:(3)4,7(8) strain genes showed significantly altered expression profiles; 344 genes were more than two-fold upregulated, and 442 genes were more than 0.5-fold downregulated. The upregulated genes were divided into the category of 21 clusters of orthologous groups (COGs), and the “not in COGs” category included 170 genes. This category had virulence plasmid genes, including the ipa-mxi-spa genes required for invasion of colorectal epithelium (type III secretion system). Quantitative reverse-transcription polymerase chain reaction results also showed the same pattern in two more atypical S. flexneri II:(3)4,7(8) strains. Atypical S. flexneri II:(3)4,7(8) showed four times increased invasion activity in Caco-2 cells than that of typical strains. CONCLUSION: Our results provide the intracellularly regulated genes that may be important for adaptation and growth strategies of this atypical S. flexneri. |
format | Online Article Text |
id | pubmed-3747661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Korea Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-37476612013-10-24 Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8) Hong, Sahyun Cha, Injun Kim, Nan-Ok Kim, Seong-Han Jung, Kyung-Tae Lee, Je-Hee Kim, Dong-Wook Park, Mi-Sun Kang, Yeon-Ho Osong Public Health Res Perspect Articles OBJECTIVES: We aimed at evaluating the virulence of atypical Shigella flexneri II:(3)4,7(8) by DNA microarray and invasion assay. METHODS: We used a customized S. flexneri DNA microarray to analyze an atypical S. flexneri II:(3)4,7(8) gene expression profile and compared it with that of the S. flexneri 2b strain. RESULTS: Approximately one-quarter of the atypical S. flexneri II:(3)4,7(8) strain genes showed significantly altered expression profiles; 344 genes were more than two-fold upregulated, and 442 genes were more than 0.5-fold downregulated. The upregulated genes were divided into the category of 21 clusters of orthologous groups (COGs), and the “not in COGs” category included 170 genes. This category had virulence plasmid genes, including the ipa-mxi-spa genes required for invasion of colorectal epithelium (type III secretion system). Quantitative reverse-transcription polymerase chain reaction results also showed the same pattern in two more atypical S. flexneri II:(3)4,7(8) strains. Atypical S. flexneri II:(3)4,7(8) showed four times increased invasion activity in Caco-2 cells than that of typical strains. CONCLUSION: Our results provide the intracellularly regulated genes that may be important for adaptation and growth strategies of this atypical S. flexneri. Korea Centers for Disease Control and Prevention 2012-12 /pmc/articles/PMC3747661/ /pubmed/24159518 http://dx.doi.org/10.1016/j.phrp.2012.10.002 Text en Copyright ©2012, Korea Centers for Disease Control and Prevention http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Hong, Sahyun Cha, Injun Kim, Nan-Ok Kim, Seong-Han Jung, Kyung-Tae Lee, Je-Hee Kim, Dong-Wook Park, Mi-Sun Kang, Yeon-Ho Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8) |
title | Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8) |
title_full | Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8) |
title_fullStr | Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8) |
title_full_unstemmed | Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8) |
title_short | Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8) |
title_sort | enhanced type iii secretion system expression of atypical shigella flexneri ii:(3)4,7(8) |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747661/ https://www.ncbi.nlm.nih.gov/pubmed/24159518 http://dx.doi.org/10.1016/j.phrp.2012.10.002 |
work_keys_str_mv | AT hongsahyun enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 AT chainjun enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 AT kimnanok enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 AT kimseonghan enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 AT jungkyungtae enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 AT leejehee enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 AT kimdongwook enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 AT parkmisun enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 AT kangyeonho enhancedtypeiiisecretionsystemexpressionofatypicalshigellaflexneriii3478 |