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Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition

BACKGROUND: Currently, there is a constant need to find microbial products for maintaining or even improving host microbiota balance that could be targeted to a selected consumer group. Blood group secretor status, determining the ABO status, could be used to stratify the consumer group. OBJECTIVE:...

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Autores principales: Mäkivuokko, Harri, Wacklin, Pirjo, Koenen, Marjorie E., Laamanen, Karoliina, Alakulppi, Noora, Venema, Koen, Mättö, Jaana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747753/
https://www.ncbi.nlm.nih.gov/pubmed/23990829
http://dx.doi.org/10.3402/mehd.v23i0.18578
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author Mäkivuokko, Harri
Wacklin, Pirjo
Koenen, Marjorie E.
Laamanen, Karoliina
Alakulppi, Noora
Venema, Koen
Mättö, Jaana
author_facet Mäkivuokko, Harri
Wacklin, Pirjo
Koenen, Marjorie E.
Laamanen, Karoliina
Alakulppi, Noora
Venema, Koen
Mättö, Jaana
author_sort Mäkivuokko, Harri
collection PubMed
description BACKGROUND: Currently, there is a constant need to find microbial products for maintaining or even improving host microbiota balance that could be targeted to a selected consumer group. Blood group secretor status, determining the ABO status, could be used to stratify the consumer group. OBJECTIVE: We have applied a validated upper intestinal tract model (TIM-1) and culturing methods to screen potential probiotic bacteria from faeces of blood secretor and non-secretor individuals. DESIGN: Faecal samples from healthy volunteers were pooled to age- and sex-matched secretor and non-secretor pools. Faecal pools were run through separate TIM-1 simulations, and bacteria were cultivated from samples taken at different stages of simulations for characterisation. RESULTS: Microbes in secretor pool survived the transit through TIM-1 system better than microbes of non-secretor pool, especially bifidobacteria and anaerobes were highly affected. The differences in numbers of bifidobacteria and lactobacilli isolates after plate cultivations and further the number of distinct RAPD-genotypes was clearly lower in non-secretor pool than in secretor pool. CONCLUSIONS: In the present study, we showed that microbiota of secretor and non-secretor individuals tolerate gastrointestinal conditions differently and that a combination of gastrointestinal simulations and cultivation methods proved to be a promising tool for isolating potentially probiotic bacteria.
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spelling pubmed-37477532013-08-29 Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition Mäkivuokko, Harri Wacklin, Pirjo Koenen, Marjorie E. Laamanen, Karoliina Alakulppi, Noora Venema, Koen Mättö, Jaana Microb Ecol Health Dis Supplement 2, 2012 BACKGROUND: Currently, there is a constant need to find microbial products for maintaining or even improving host microbiota balance that could be targeted to a selected consumer group. Blood group secretor status, determining the ABO status, could be used to stratify the consumer group. OBJECTIVE: We have applied a validated upper intestinal tract model (TIM-1) and culturing methods to screen potential probiotic bacteria from faeces of blood secretor and non-secretor individuals. DESIGN: Faecal samples from healthy volunteers were pooled to age- and sex-matched secretor and non-secretor pools. Faecal pools were run through separate TIM-1 simulations, and bacteria were cultivated from samples taken at different stages of simulations for characterisation. RESULTS: Microbes in secretor pool survived the transit through TIM-1 system better than microbes of non-secretor pool, especially bifidobacteria and anaerobes were highly affected. The differences in numbers of bifidobacteria and lactobacilli isolates after plate cultivations and further the number of distinct RAPD-genotypes was clearly lower in non-secretor pool than in secretor pool. CONCLUSIONS: In the present study, we showed that microbiota of secretor and non-secretor individuals tolerate gastrointestinal conditions differently and that a combination of gastrointestinal simulations and cultivation methods proved to be a promising tool for isolating potentially probiotic bacteria. Co-Action Publishing 2012-06-18 /pmc/articles/PMC3747753/ /pubmed/23990829 http://dx.doi.org/10.3402/mehd.v23i0.18578 Text en © 2012 Harri Mäkivuokko et al. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Supplement 2, 2012
Mäkivuokko, Harri
Wacklin, Pirjo
Koenen, Marjorie E.
Laamanen, Karoliina
Alakulppi, Noora
Venema, Koen
Mättö, Jaana
Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
title Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
title_full Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
title_fullStr Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
title_full_unstemmed Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
title_short Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
title_sort isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
topic Supplement 2, 2012
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747753/
https://www.ncbi.nlm.nih.gov/pubmed/23990829
http://dx.doi.org/10.3402/mehd.v23i0.18578
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