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Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining

The primo-vascular system (PVS), which consists of primo-vessels (PVs) and primo-nodes (PNs), is a novel thread-like structure identified in many animal species. Various observational methods have been used to clarify its anatomical properties. Here, we used Hemacolor staining to examine the gross m...

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Autores principales: Lim, Chae Jeong, Yoo, Jong-Hyun, Kim, Yongbaek, Lee, So Yeong, Ryu, Pan Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748414/
https://www.ncbi.nlm.nih.gov/pubmed/23986781
http://dx.doi.org/10.1155/2013/350815
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author Lim, Chae Jeong
Yoo, Jong-Hyun
Kim, Yongbaek
Lee, So Yeong
Ryu, Pan Dong
author_facet Lim, Chae Jeong
Yoo, Jong-Hyun
Kim, Yongbaek
Lee, So Yeong
Ryu, Pan Dong
author_sort Lim, Chae Jeong
collection PubMed
description The primo-vascular system (PVS), which consists of primo-vessels (PVs) and primo-nodes (PNs), is a novel thread-like structure identified in many animal species. Various observational methods have been used to clarify its anatomical properties. Here, we used Hemacolor staining to examine the gross morphology of organ-surface PVS in rats. We observed a sinus structure (20–50 μm) with a remarkably low cellularity within PNs and PVs and several lines of ductules (3–5 μm) filled with single cells or granules (~1 μm) in PV. Both sinuses and ductules were linearly aligned along the longitudinal axis of the PVS. Such morphology of the PVS was further confirmed by acridine orange staining. In PN slices, there was a honeycomb-like structure containing the granules with pentagonal lumens (~10 μm). Both PVs and PNs were densely filled with WBCs, RBCs, and putative mast cells (MCs), which were 90.3%, 5.9%, and 3.8% of the cell population, respectively. Granules in putative MCs showed spontaneous vibrating movements. In conclusion, the results show that Hemacolor, a simple and rapid staining system, can reveal the gross morphological features reported previously. Our findings may help to elucidate the structure and function of the PVS in normal and disease states in future studies.
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spelling pubmed-37484142013-08-28 Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining Lim, Chae Jeong Yoo, Jong-Hyun Kim, Yongbaek Lee, So Yeong Ryu, Pan Dong Evid Based Complement Alternat Med Research Article The primo-vascular system (PVS), which consists of primo-vessels (PVs) and primo-nodes (PNs), is a novel thread-like structure identified in many animal species. Various observational methods have been used to clarify its anatomical properties. Here, we used Hemacolor staining to examine the gross morphology of organ-surface PVS in rats. We observed a sinus structure (20–50 μm) with a remarkably low cellularity within PNs and PVs and several lines of ductules (3–5 μm) filled with single cells or granules (~1 μm) in PV. Both sinuses and ductules were linearly aligned along the longitudinal axis of the PVS. Such morphology of the PVS was further confirmed by acridine orange staining. In PN slices, there was a honeycomb-like structure containing the granules with pentagonal lumens (~10 μm). Both PVs and PNs were densely filled with WBCs, RBCs, and putative mast cells (MCs), which were 90.3%, 5.9%, and 3.8% of the cell population, respectively. Granules in putative MCs showed spontaneous vibrating movements. In conclusion, the results show that Hemacolor, a simple and rapid staining system, can reveal the gross morphological features reported previously. Our findings may help to elucidate the structure and function of the PVS in normal and disease states in future studies. Hindawi Publishing Corporation 2013 2013-08-06 /pmc/articles/PMC3748414/ /pubmed/23986781 http://dx.doi.org/10.1155/2013/350815 Text en Copyright © 2013 Chae Jeong Lim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lim, Chae Jeong
Yoo, Jong-Hyun
Kim, Yongbaek
Lee, So Yeong
Ryu, Pan Dong
Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining
title Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining
title_full Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining
title_fullStr Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining
title_full_unstemmed Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining
title_short Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining
title_sort gross morphological features of the organ surface primo-vascular system revealed by hemacolor staining
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748414/
https://www.ncbi.nlm.nih.gov/pubmed/23986781
http://dx.doi.org/10.1155/2013/350815
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