Cargando…

Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae

Metal nanosolicoparticles are suspected to cause diseases in a number of organisms, including man. In this paper, we report the effects of nanosilver (Ag, 1–20 nm particles) on the early development of the zebrafish, a well-established vertebrate model. Embryos at the midgastrula stage were exposed...

Descripción completa

Detalles Bibliográficos
Autores principales: Myrzakhanova, Marzhan, Gambardella, Chiara, Falugi, Carla, Gatti, Antonietta M., Tagliafierro, Grazia, Ramoino, Paola, Bianchini, Paolo, Diaspro, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748442/
https://www.ncbi.nlm.nih.gov/pubmed/23991412
http://dx.doi.org/10.1155/2013/205183
_version_ 1782281069238681600
author Myrzakhanova, Marzhan
Gambardella, Chiara
Falugi, Carla
Gatti, Antonietta M.
Tagliafierro, Grazia
Ramoino, Paola
Bianchini, Paolo
Diaspro, Alberto
author_facet Myrzakhanova, Marzhan
Gambardella, Chiara
Falugi, Carla
Gatti, Antonietta M.
Tagliafierro, Grazia
Ramoino, Paola
Bianchini, Paolo
Diaspro, Alberto
author_sort Myrzakhanova, Marzhan
collection PubMed
description Metal nanosolicoparticles are suspected to cause diseases in a number of organisms, including man. In this paper, we report the effects of nanosilver (Ag, 1–20 nm particles) on the early development of the zebrafish, a well-established vertebrate model. Embryos at the midgastrula stage were exposed to concentrations ranging from 100 to 0.001 mg/L to verify the effects on different endpoints: lethality, morphology, expression of cholinergic molecules, and development of the immune system. (1) Relative risk of mortality was exponential in the range between 0.001 and 10 mg/L. Exposure to 100 mg/L caused 100% death of embryos before reaching the tail-bud stage. (2) Developmental anomalies were present in the 72 h larvae obtained from embryos exposed to nanosilver: whole body length, decreased eye dimension, and slow response to solicitation by gentle touch with a needle tip, with a significant threshold at 0.1 mg/L. (3) Dose-dependent inhibition of acetylcholinesterase activity was significant among the exposures, except between 1 mg/L and 10 mg/L. (4) The distribution of CD41+ cells and of CDF/LIF-like immunoreactivity was altered according to the Ag concentration. The possible effect of nanosilver in impairing immune system differentiation through the inhibition of molecules related to the cholinergic system is discussed.
format Online
Article
Text
id pubmed-3748442
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-37484422013-08-29 Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae Myrzakhanova, Marzhan Gambardella, Chiara Falugi, Carla Gatti, Antonietta M. Tagliafierro, Grazia Ramoino, Paola Bianchini, Paolo Diaspro, Alberto Biomed Res Int Research Article Metal nanosolicoparticles are suspected to cause diseases in a number of organisms, including man. In this paper, we report the effects of nanosilver (Ag, 1–20 nm particles) on the early development of the zebrafish, a well-established vertebrate model. Embryos at the midgastrula stage were exposed to concentrations ranging from 100 to 0.001 mg/L to verify the effects on different endpoints: lethality, morphology, expression of cholinergic molecules, and development of the immune system. (1) Relative risk of mortality was exponential in the range between 0.001 and 10 mg/L. Exposure to 100 mg/L caused 100% death of embryos before reaching the tail-bud stage. (2) Developmental anomalies were present in the 72 h larvae obtained from embryos exposed to nanosilver: whole body length, decreased eye dimension, and slow response to solicitation by gentle touch with a needle tip, with a significant threshold at 0.1 mg/L. (3) Dose-dependent inhibition of acetylcholinesterase activity was significant among the exposures, except between 1 mg/L and 10 mg/L. (4) The distribution of CD41+ cells and of CDF/LIF-like immunoreactivity was altered according to the Ag concentration. The possible effect of nanosilver in impairing immune system differentiation through the inhibition of molecules related to the cholinergic system is discussed. Hindawi Publishing Corporation 2013 2013-08-06 /pmc/articles/PMC3748442/ /pubmed/23991412 http://dx.doi.org/10.1155/2013/205183 Text en Copyright © 2013 Marzhan Myrzakhanova et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Myrzakhanova, Marzhan
Gambardella, Chiara
Falugi, Carla
Gatti, Antonietta M.
Tagliafierro, Grazia
Ramoino, Paola
Bianchini, Paolo
Diaspro, Alberto
Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae
title Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae
title_full Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae
title_fullStr Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae
title_full_unstemmed Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae
title_short Effects of Nanosilver Exposure on Cholinesterase Activities, CD41, and CDF/LIF-Like Expression in ZebraFish (Danio rerio) Larvae
title_sort effects of nanosilver exposure on cholinesterase activities, cd41, and cdf/lif-like expression in zebrafish (danio rerio) larvae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748442/
https://www.ncbi.nlm.nih.gov/pubmed/23991412
http://dx.doi.org/10.1155/2013/205183
work_keys_str_mv AT myrzakhanovamarzhan effectsofnanosilverexposureoncholinesteraseactivitiescd41andcdfliflikeexpressioninzebrafishdanioreriolarvae
AT gambardellachiara effectsofnanosilverexposureoncholinesteraseactivitiescd41andcdfliflikeexpressioninzebrafishdanioreriolarvae
AT falugicarla effectsofnanosilverexposureoncholinesteraseactivitiescd41andcdfliflikeexpressioninzebrafishdanioreriolarvae
AT gattiantoniettam effectsofnanosilverexposureoncholinesteraseactivitiescd41andcdfliflikeexpressioninzebrafishdanioreriolarvae
AT tagliafierrograzia effectsofnanosilverexposureoncholinesteraseactivitiescd41andcdfliflikeexpressioninzebrafishdanioreriolarvae
AT ramoinopaola effectsofnanosilverexposureoncholinesteraseactivitiescd41andcdfliflikeexpressioninzebrafishdanioreriolarvae
AT bianchinipaolo effectsofnanosilverexposureoncholinesteraseactivitiescd41andcdfliflikeexpressioninzebrafishdanioreriolarvae
AT diasproalberto effectsofnanosilverexposureoncholinesteraseactivitiescd41andcdfliflikeexpressioninzebrafishdanioreriolarvae