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Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis
Background: Auto-focusing is an important operation in high throughput imaging scanning. Although many auto-focusing methods have been developed and tested for a variety of imaging modalities, few investigations have been performed on the selection of an optimal auto-focusing method that is suitable...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748595/ https://www.ncbi.nlm.nih.gov/pubmed/23629477 http://dx.doi.org/10.3233/ACP-130077 |
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author | Qiu, Yuchen Chen, Xiaodong Li, Yuhua Chen, Wei R. Zheng, Bin Li, Shibo Liu, Hong |
author_facet | Qiu, Yuchen Chen, Xiaodong Li, Yuhua Chen, Wei R. Zheng, Bin Li, Shibo Liu, Hong |
author_sort | Qiu, Yuchen |
collection | PubMed |
description | Background: Auto-focusing is an important operation in high throughput imaging scanning. Although many auto-focusing methods have been developed and tested for a variety of imaging modalities, few investigations have been performed on the selection of an optimal auto-focusing method that is suitable for the pathological metaphase chromosome analysis under a high resolution scanning microscopic system. Objective: The purpose of this study is to investigate and identify an optimal auto-focusing method for the pathological metaphase chromosome analysis. Methods: In this study, five auto-focusing methods were applied and tested using metaphase chromosome images acquired from bone marrow and blood specimens. These methods were assessed by measuring a number of indices including execution time, accuracy, number of false maxima, and full width at half maximum (FWHM). Results: For the specific condition investigated in this study, the results showed that the Brenner gradient and threshold pixel counting methods were the optimal methods for acquiring high quality metaphase chromosome images from the bone marrow and blood specimens, respectively. Conclusions: Selecting an optimal auto-focusing method depends on the specific clinical tasks. This study also provides useful information for the design and implementation of the high throughput microscopic image scanning systems in the future digital pathology. |
format | Online Article Text |
id | pubmed-3748595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-37485952014-01-01 Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis Qiu, Yuchen Chen, Xiaodong Li, Yuhua Chen, Wei R. Zheng, Bin Li, Shibo Liu, Hong Anal Cell Pathol (Amst) Other Background: Auto-focusing is an important operation in high throughput imaging scanning. Although many auto-focusing methods have been developed and tested for a variety of imaging modalities, few investigations have been performed on the selection of an optimal auto-focusing method that is suitable for the pathological metaphase chromosome analysis under a high resolution scanning microscopic system. Objective: The purpose of this study is to investigate and identify an optimal auto-focusing method for the pathological metaphase chromosome analysis. Methods: In this study, five auto-focusing methods were applied and tested using metaphase chromosome images acquired from bone marrow and blood specimens. These methods were assessed by measuring a number of indices including execution time, accuracy, number of false maxima, and full width at half maximum (FWHM). Results: For the specific condition investigated in this study, the results showed that the Brenner gradient and threshold pixel counting methods were the optimal methods for acquiring high quality metaphase chromosome images from the bone marrow and blood specimens, respectively. Conclusions: Selecting an optimal auto-focusing method depends on the specific clinical tasks. This study also provides useful information for the design and implementation of the high throughput microscopic image scanning systems in the future digital pathology. IOS Press 2013 2013-04-29 /pmc/articles/PMC3748595/ /pubmed/23629477 http://dx.doi.org/10.3233/ACP-130077 Text en Copyright © 2013 Hindawi Publishing Corporation and the authors. |
spellingShingle | Other Qiu, Yuchen Chen, Xiaodong Li, Yuhua Chen, Wei R. Zheng, Bin Li, Shibo Liu, Hong Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis |
title | Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis |
title_full | Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis |
title_fullStr | Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis |
title_full_unstemmed | Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis |
title_short | Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis |
title_sort | evaluations of auto-focusing methods under a microscopic imaging modality for metaphase chromosome image analysis |
topic | Other |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748595/ https://www.ncbi.nlm.nih.gov/pubmed/23629477 http://dx.doi.org/10.3233/ACP-130077 |
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